RNAi: a powerful tool to unravel hepatitis C virus-host interactions within the infectious life cycle

Journal of Hepatology
Joachim LupbergerThomas F Baumert

Abstract

Cellular cofactors affecting hepatitis C virus infection and replication. Randall G, Panis M, Cooper JD, Tellinghuisen TL, Sukhodolets KE, Pfeffer S, Landthaler M, Landgraf P, Kan S, Lindenbach BD, Chien M, Weir DB, Russo JJ, Ju J, Brownstein MJ, Sheridan R, Sander C, Zavolan M, Tuschl T, Rice CM. Recently identified hepatitis C virus (HCV) isolates that are infectious in cell culture provide a genetic system to evaluate the significance of virus-host interactions for HCV replication. We have completed a systematic RNAi screen wherein siRNAs were designed that target 62 host genes encoding proteins that physically interact with HCV RNA or proteins or belong to cellular pathways thought to modulate HCV infection. This includes 10 host proteins that we identify in this study to bind HCV NS5A. siRNAs that target 26 of these host genes alter infectious HCV production >3-fold. Included in this set of 26 were siRNAs that target DICER, a principal component of the RNAi silencing pathway. Contrary to the hypothesis that RNAi is an antiviral pathway in mammals, as has been reported for subgenomic HCV replicons, siRNAs that target DICER inhibited HCV replication. Furthermore, siRNAs that target several other components of the RNAi pathwa...Continue Reading

References

Jun 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jin ZhongFrancis V Chisari
Jun 11, 2005·Science·Brett D LindenbachCharles M Rice
Sep 6, 2005·Science·Catherine L JoplingPeter Sarnow
Jun 29, 2006·Cellular Microbiology·Eva-Maria Damm, Lucas Pelkmans
Aug 17, 2006·Nature Reviews. Microbiology·Peter SarnowKaren A Wehner
May 9, 2007·Gastroenterology·Jean-Michel PawlotskyJohn G McHutchison
May 24, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Teresa I NgAkhteruzzaman Molla
Jul 10, 2007·Proceedings of the National Academy of Sciences of the United States of America·Glenn RandallCharles M Rice
Nov 16, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Mirjam B ZeiselThomas F Baumert

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Citations

May 26, 2009·Nature Biotechnology·Aly A KhanDebora S Marks
Mar 16, 2011·Mutation Research·Alton EtheridgeKai Wang
Apr 20, 2010·Journal of Virological Methods·Robin KettelerBrian Seed
Oct 8, 2009·Journal of Basic Microbiology·Qun-Jun DuanShi-Qiang Shang
Aug 14, 2012·Virus Research·Yu-Jiao YangXian-Zhu Xia
Aug 27, 2010·Molecular Systems Biology·Julja BurchardJohn M Luk
Jun 21, 2014·Journal of Nanobiotechnology·Zhiwei MaShan Gao

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