RNAi-based strategies for cyclooxygenase-2 inhibition in cancer.

Journal of Biomedicine & Biotechnology
Antonio StrillacciEnzo Spisni

Abstract

Cyclooxygenase-2 (COX-2) enzyme has been involved in the tumorigenesis and in the progression of colorectal cancer (CRC). The use of traditional nonsteroidal anti-inflammatory drugs (NSAIDs) or selective COX-2 inhibitors has been proposed for the prevention and the treatment of this relevant neoplastic disease. In the light of an innovative alternative to these pharmacological approaches, we review here the possible strategies to achieve a strong and selective inhibition of COX-2 enzyme by using the mechanism of RNA Interference (RNAi) targeted against its mRNA. Anti-COX-2 siRNA molecules (siCOX-2) can be generated in CRC cells from short hairpin RNA (shRNA) precursors, delivered in vitro by a retroviral expression system, and induce a significant and stable silencing of overexpressed COX-2 in human colon cancer cells. As a safer alternative to viral approach, nonpathogenic bacteria (E. coli) can be engineered to invade eukaryotic cells and to generate siCOX-2 molecules in cancer cells. Moreover, the involvement of miRNAs in COX-2 posttranscriptional regulation opens up the possibility to exploit an endogenous silencing mechanism to knockdown overexpressed COX-2. Thus, these recent strategies disclose new challenging perspectiv...Continue Reading

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Jan 22, 2011·Journal of Biomedicine & Biotechnology·Martina Perše, Anton Cerar
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Nov 14, 2014·Molecular Therapy : the Journal of the American Society of Gene Therapy·Enzo SpisniAntonio Strillacci
Aug 15, 2021·Clinica Chimica Acta; International Journal of Clinical Chemistry·Wanjun LiSongkai Long

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BETA
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