RNAi-mediated knockdown of FANCF suppresses cell proliferation, migration, invasion, and drug resistance potential of breast cancer cells

Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas
L ZhaoM J Wei

Abstract

Fanconi anemia complementation group F protein (FANCF) is a key factor, which maintains the function of FA/BRCA, a DNA damage response pathway. However, the functional role of FANCF in breast cancer has not been elucidated. We performed a specific FANCF-shRNA knockdown of endogenous FANCF in vitro. Cell viability was measured with a CCK-8 assay. DNA damage was assessed with an alkaline comet assay. Apoptosis, cell cycle, and drug accumulation were measured by flow cytometry. The expression levels of protein were determined by Western blot using specific antibodies. Based on these results, we used cell migration and invasion assays to demonstrate a crucial role for FANCF in those processes. FANCF shRNA effectively inhibited expression of FANCF. We found that proliferation of FANCF knockdown breast cancer cells (MCF-7 and MDA-MB-435S) was significantly inhibited, with cell cycle arrest in the S phase, induction of apoptosis, and DNA fragmentation. Inhibition of FANCF also resulted in decreased cell migration and invasion. In addition, FANCF knockdown enhanced sensitivity to doxorubicin in breast cancer cells. These results suggest that FANCF may be a potential target for molecular, therapeutic intervention in breast cancer.

References

Mar 1, 1988·Experimental Cell Research·N P SinghE L Schneider
Jun 12, 1996·Mutation Research·M KlaudeG Ahnström
Mar 10, 2001·Molecular Cell·I Garcia-HigueraA D D'Andrea
Jun 5, 2003·Cancer Cell·Olufunmilayo I Olopade, Minjie Wei
Nov 18, 2003·British Journal of Haematology·Marc TischkowitzHans Joenje
May 6, 2004·Cancer Research·Gopeshwar NarayanVundavalli V V S Murty
Jul 21, 2004·The Journal of Biological Chemistry·France LéveilléJohan P de Winter
Dec 27, 2005·Cell·Laura J NiedernhoferJan H J Hoeijmakers
May 3, 2006·Cancer Research·Lonnie P SwiftSuzanne M Cutts
Nov 4, 2006·The Journal of Biological Chemistry·Przemyslaw KowalTom Ellenberger
Jan 16, 2007·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Clark C ChenAlan D'Andrea
May 2, 2007·Cell Cycle·Alex Lyakhovich, Jordi Surrallés
Oct 13, 2007·Breast Cancer Research and Treatment·Minjie WeiOlufunmilayo I Olopade
Nov 15, 2007·Cytogenetic and Genome Research·K NevelingD Schindler
Apr 17, 2008·British Journal of Cancer·S L LimJ A Green
Jul 2, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Alan Ashworth
Jul 23, 2009·Mutation Research·Arleen D Auerbach
Oct 9, 2009·Breast Cancer : the Journal of the Japanese Breast Cancer Society·Eriko TokunagaYoshihiko Maehara
Apr 20, 2010·Nature Genetics·Fiona VazChristopher G Mathew
May 21, 2010·The New England Journal of Medicine·Alan D D'Andrea
Dec 17, 2010·Molecular Cancer·Arti ShuklaBrooke T Mossman
Jan 18, 2011·Nature Genetics·Yonghwan KimAgata Smogorzewska
Feb 8, 2011·CA: a Cancer Journal for Clinicians·Ahmedin JemalDavid Forman
Mar 16, 2011·Physiological Research·T Hucl, E Gallmeier
Oct 3, 2012·Bioorganic & Medicinal Chemistry·Tomoe KusayanagiFumio Sugawara

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Methods Mentioned

BETA
transfection
electrophoresis
Assay
FCS
ubiquitination
flow cytometry
fluorescence-activated cell sorting

Software Mentioned

SPSS
Comet Assay Software Project ( CASP )

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