RNAi-mediated silencing of ABCD3 gene expression in rat C6 glial cells: a model system to study PMP70 function

Neurochemistry International
Rita Di BenedettoAntonella Di Biase

Abstract

The function of PMP70, one of the four ABC half-transporters of mammalian peroxisomes, encoded by ABCD3 gene, is still unclear. The finding that PMP70 over-expression partially corrected very long-chain fatty acid oxidation defects in fibroblasts of X-linked adrenoleukodystrophy patients, has unveiled its potential clinical relevance, prompting us to set up a model system to study PMP70 function. We used the RNA interference technique, a powerful approach to loss-of-function gene expression analysis, to knockdown the ABCD3 gene in the rat glial C6 cell line, since glia could represent the target tissue of X-linked adrenoleukodystrophy disease. Cells were transfected with a vector for RNA interference generating small interfering RNAs that specifically target the ABCD3 mRNA. By using a puromycin-selectable version of the plasmid, we generated a stable cell line (abcd3kd), in which we observed a stable decrease of PMP70 protein expression greater than 70%. We thus examined the effect of ABCD3 knockdown on lignoceric and palmitic acids beta-oxidation and we found that in abcd3kd cells the rate of peroxisomal and mitochondrial beta-oxidation activities were both reduced about one-third compared with control cells. The mitochondrial...Continue Reading

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Citations

Mar 15, 2012·Histochemistry and Cell Biology·Markus IslingerMichael Schrader
Nov 11, 2008·Neurochemistry International·Rita Di BenedettoAntonella Di Biase
Nov 5, 2013·Computer Methods and Programs in Biomedicine·Bin HanShen Wang
Mar 30, 2010·Biochimica Et Biophysica Acta·M IslingerM Schrader
Aug 30, 2014·Human Molecular Genetics·Sacha FerdinandusseDavid Valle
Nov 1, 2015·Biochemical Society Transactions·Alison BakerFrederica L Theodoulou
Jun 14, 2019·Molecular Syndromology·Akella Radha Rama DeviShaik M Naushad

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