RNAi-mediated tocopherol deficiency impairs photoassimilate export in transgenic potato plants

Plant Physiology
Daniel HofiusUwe Sonnewald

Abstract

Tocopherols (vitamin E) are lipophilic antioxidants presumed to play a key role in protecting chloroplast membranes and the photosynthetic apparatus from photooxidative damage. Additional nonantioxidant functions of tocopherols have been proposed after the recent finding that the Suc export defective1 maize (Zea mays) mutant (sxd1) carries a defect in tocopherol cyclase (TC) and thus is devoid of tocopherols. However, the corresponding vitamin E deficient1 Arabidopsis mutant (vte1) lacks a phenotype analogous to sxd1, suggesting differences in tocopherol function between C4 and C3 plants. Therefore, in this study, the potato (Solanum tuberosum) ortholog of SXD1 was isolated and functionally characterized. StSXD1 encoded a protein with high TC activity in vitro, and chloroplastic localization was demonstrated by transient expression of green fluorescent protein-tagged fusion constructs. RNAi-mediated silencing of StSXD1 in transgenic potato plants resulted in the disruption of TC activity and severe tocopherol deficiency similar to the orthologous sxd1 and vte1 mutants. The nearly complete absence of tocopherols caused a characteristic photoassimilate export-defective phenotype comparable to sxd1, which appeared to be a conseque...Continue Reading

References

Jul 1, 1992·The Plant Journal : for Cell and Molecular Biology·U Sonnewald
Apr 1, 1990·Plant Molecular Biology·R Johnson, C A Ryan
Nov 30, 1990·Biochimica Et Biophysica Acta·B D McKersieL C Krieg
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Feb 11, 1994·Science·M ChalfieD C Prasher
Mar 6, 1999·Current Opinion in Plant Biology·K Herbers, U Sonnewald
Feb 22, 2001·Journal of Experimental Botany·F Schaller
May 23, 2002·FEBS Letters·Angelo AzziJean Marc Zingg
Jun 5, 2002·The Plant Cell·Filip RollandJen Sheen
Jun 5, 2002·The Plant Cell·Valerie HaywoodWilliam J Lucas
Jun 26, 2002·Plant Molecular Biology·Lars OstergaardJohn Mundy
Sep 6, 2002·Proceedings of the National Academy of Sciences of the United States of America·Svetlana PorfirovaPeter Dormann
Jan 1, 2003·Journal of Experimental Botany·Matthew J Paul, Till K Pellny
Apr 15, 2003·The Proceedings of the Nutrition Society·Gerald RimbachPeter D Weinberg
Aug 16, 2003·Science·Marc T NishimuraShauna C Somerville
Oct 14, 2003·The Plant Cell·Andrew K JacobsGeoffrey B Fincher
Oct 31, 2003·Planta·Sergi Munné-Bosch, Jon Falk
Nov 18, 2003·The Plant Journal : for Cell and Molecular Biology·Shuai ChenFrederik Börnke
Nov 25, 2003·The Plant Cell·Alison L Van EenennaamRobert L Last
Dec 20, 2003·Plant Molecular Biology·Eveline BergmüllerPeter Dörmann
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Paul M. HasegawaHans J. Bohnert

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Citations

Nov 23, 2006·Planta·Peter Dörmann
Nov 26, 2010·Protoplasma·Yoselin Benitez-AlfonsoAndy Maule
Apr 7, 2007·Transgenic Research·Elizabeth F CrowellDavid S Douches
May 30, 2013·Journal of Agricultural and Food Chemistry·Nazim HussainLixi Jiang
Sep 15, 2010·Proceedings of the National Academy of Sciences of the United States of America·Laurent Mène-SaffranéDean DellaPenna
Apr 14, 2010·Journal of Experimental Botany·Jon Falk, Sergi Munné-Bosch
May 4, 2006·Annual Review of Plant Biology·Dean DellaPenna, Barry J Pogson
Mar 5, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Barbara Demmig-AdamsWilliam W Adams
Mar 31, 2015·Ukrainskiĭ biokhimicheskiĭ zhurnal·V M Mokrosnop
Dec 29, 2009·Plant Physiology and Biochemistry : PPB·Laurent Mène-Saffrané, Dean DellaPenna
Nov 21, 2007·Plant Physiology and Biochemistry : PPB·Renata Szymańska, Jerzy Kruk
Apr 17, 2007·Current Opinion in Plant Biology·Hiroshi Maeda, Dean DellaPenna
Mar 13, 2016·New Biotechnology·Jerzy KrukJolanta Dłużewska
Oct 12, 2015·Journal of Plant Physiology·Gregorio Gálvez-ValdiviesoMiguel Aguilar
Apr 12, 2005·The New Phytologist·Sergi Munne-Bosch
Aug 9, 2013·Plant Biotechnology Journal·Philippa J BarrellAnthony J Conner
Nov 17, 2007·Biochimica Et Biophysica Acta·Jerzy Kruk, Achim Trebst

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