RNAi screen identifies MAPK14 as a druggable suppressor of human hematopoietic stem cell expansion.

Blood
Aurélie BaudetJonas Larsson

Abstract

We report on a forward RNAi screen in primary human hematopoietic stem and progenitor cells, using pooled lentiviral shRNA libraries deconvoluted by next generation sequencing. We identify MAPK14/p38α as a modulator of ex vivo stem cell proliferation and show that pharmacologic inhibition of p38 dramatically enhances the stem cell activity of cultured umbilical cord blood derived hematopoietic cells. p38 inhibitors should thus be considered in strategies aiming at expanding stem cells for clinical benefit.

References

Nov 7, 2006·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Gerard J MadlambayanPeter W Zandstra
Oct 6, 2009·Bone Marrow Transplantation·H E BroxmeyerG Hangoc
Mar 23, 2010·Nature Medicine·Heather A HimburgJohn P Chute
Aug 6, 2010·Methods in Molecular Biology·Christine KarlssonAurélie Baudet
Oct 5, 2010·Cell Stem Cell·Song ChouHarvey Lodish
Mar 26, 2011·Blood·Ann DahlbergIrwin D Bernstein

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Citations

Mar 16, 2013·Cell Death & Disease·Kui WangYuquan Wei
May 9, 2014·Current Opinion in Hematology·Christine KarlssonJonas Larsson
Jul 30, 2014·Stem Cell Reports·Frederic B ThalheimerMichael A Rieger
Mar 13, 2015·Blood·Susumu GoyamaJames C Mulloy
Apr 1, 2015·The Journal of Experimental Medicine·Melania TesioAndreas Trumpp
Jul 15, 2017·Antioxidants & Redox Signaling·Darren Q Tan, Toshio Suda
Mar 23, 2021·The Journal of Biological Chemistry·Yuriko SorimachiKeiyo Takubo
Jul 3, 2021·Antioxidants·Tullia MaraldiSilvana Hrelia

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