RNAi suppression of rice endogenous storage proteins enhances the production of rice-based Botulinum neutrotoxin type A vaccine

Vaccine
Yoshikazu YukiHiroshi Kiyono

Abstract

Mucosal vaccines based on rice (MucoRice) offer a highly practical and cost-effective strategy for vaccinating large populations against mucosal infections. However, the limitation of low expression and yield of vaccine antigens with high molecular weight remains to be overcome. Here, we introduced RNAi technology to advance the MucoRice system by co-introducing antisense sequences specific for genes encoding endogenous rice storage proteins to minimize storage protein production and allow more space for the accumulation of vaccine antigen in rice seed. When we used RNAi suppression of a combination of major rice endogenous storage proteins, 13 kDa prolamin and glutelin A in a T-DNA vector, we could highly express a vaccine comprising the 45 kDa C-terminal half of the heavy chain of botulinum type A neurotoxin (BoHc), at an average of 100 μg per seed (MucoRice-BoHc). The MucoRice-Hc was water soluble, and was expressed in the cytoplasm but not in protein body I or II of rice seeds. Thus, our adaptation of the RNAi system improved the yield of a vaccine antigen with a high molecular weight. When the mucosal immunogenicity of the purified MucoRice-BoHc was examined, the vaccine induced protective immunity against a challenge with...Continue Reading

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Citations

Jan 10, 2014·Planta·Satyajit SaurabhDinesh Prasad
May 11, 2013·Expert Review of Vaccines·Robert P Webb, Leonard A Smith
Feb 7, 2016·Regulatory Toxicology and Pharmacology : RTP·Yoshikazu YukiHiroshi Kiyono
Jun 24, 2015·Plant Biotechnology Journal·Fumio TakaiwaTakachika Hiroi
Sep 22, 2015·Plant Biotechnology Journal·Stephen J StreatfieldVidadi Yusibov
Oct 5, 2013·Biotechnology Journal·Yuhya Wakasa, Fumio Takaiwa
Sep 2, 2014·Bioscience, Biotechnology, and Biochemistry·Masaharu Kuroda, Sachiko Ikenaga
Dec 3, 2014·Vaccine·Aayam LamichhaneHiroshi Kiyonoa
Jul 17, 2014·Archivum Immunologiae Et Therapiae Experimentalis·Tatsuhiko AzegamiYoshikazu Yuki
Aug 5, 2014·Expert Review of Anti-infective Therapy·Fabrizio AnniballiCarlo Alessandro Locatelli
Dec 17, 2015·Therapeutic Advances in Vaccines·Natsumi TakeyamaYoshikazu Yuki
Aug 9, 2013·The Journal of Clinical Investigation·Daisuke TokuharaYoshikazu Yuki
Apr 3, 2016·British Journal of Clinical Pharmacology·Matilde MerlinLinda Avesani
Mar 2, 2021·Transgenic Research·J F BuyelL Bortesi

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Proceedings of the National Academy of Sciences of the United States of America
Tomonori NochiHiroshi Kiyono
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