RNAi targeting putative genes in phosphatidylcholine turnover results in significant change in fatty acid composition in Crambe abyssinica seed oil

Lipids
Rui GuanLi-Hua Zhu

Abstract

The aim of this study was to evaluate the importance of three enzymes, LPCAT, PDCT and PDAT, involved in acyl turnover in phosphatidylcholine in order to explore the possibility of further increasing erucic acid (22:1) content in Crambe seed oil. The complete coding sequences of LPCAT1-1 and LPCAT1-2 encoding lysophosphatidylcholine acyltransferase (LPCAT), PDCT1 and PDCT2 encoding phosphatidylcholine:diacylglycerol cholinephosphotransferase (PDCT), and PDAT encoding phospholipid:diacylglycerol acyltransferase (PDAT) were cloned from developing Crambe seeds. The alignment of deduced amino acid sequences displayed a high similarity to the Arabidopsis homologs. Transgenic lines expressing RNA interference (RNAi) targeting either single or double genes showed significant changes in the fatty acid composition of seed oil. An increase in oleic acid (18:1) was observed, to varying degrees, in all of the transgenic lines, and a cumulative effect of increased 18:1 was shown in the LPCAT-PDCT double-gene RNAi. However, LPCAT single-gene RNAi led to a decrease in 22:1 accumulation, while PDCT or PDAT single-gene RNAi had no obvious effect on the level of 22:1. In agreement with the abovementioned oil phenotypes, the transcript levels of ...Continue Reading

References

Sep 11, 1998·Plant Physiology·X BaoJ Ohlrogge
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·A DahlqvistS Stymne
Feb 21, 2004·Plant Physiology·Hyun Uk Kim, Anthony H C Huang
Feb 3, 2007·Lipids·Shiu-Cheung Lung, Randall J Weselake
May 15, 2008·The Plant Journal : for Cell and Molecular Biology·John M DyerAnders S Carlsson
Apr 14, 2009·Biochemical and Biophysical Research Communications·Hideo ShindouTakao Shimizu
Oct 17, 2009·Proceedings of the National Academy of Sciences of the United States of America·Chaofu LuJohn Browse
Nov 22, 2011·European Journal of Lipid Science and Technology : EJLST·Anders S CarlssonPer Hofvander
May 31, 2012·Plant Biotechnology Journal·Xueyuan LiLi-Hua Zhu
Mar 27, 2013·Current Opinion in Plant Biology·Philip D BatesJohn Ohlrogge

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Citations

Jul 2, 2020·Critical Reviews in Biotechnology·Weicong QiHongbo Shao

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