RNAs are packaged into human cytomegalovirus virions in proportion to their intracellular concentration

Journal of Virology
Scott S TerhuneThomas Shenk

Abstract

The assembly of human cytomegalovirus (HCMV) virions is a complex process and involves the incorporation of viral transcripts. These RNAs are delivered to the newly infected cells and have the potential to be translated in the absence of HCMV gene expression. We have quantified the relative amount of RNAs in HCMV virions and infected cells with real-time reverse transcription-PCR and observed that viral and cellular RNAs are packaged in proportion to the amount of RNA within the cell at the time of assembly. To determine whether cis elements influenced RNA packaging, we constructed a recombinant HCMV mutant virus that expressed the yellow fluorescence protein (YFP) gene fused to the virion RNA UL21.5. We also constructed a mutant virus in which the UL21.5 transcription unit was replaced with the YFP gene. YFP RNA was incorporated into both viruses, indicating that RNA is incorporated in the absence of a virus-specific signal motif. Furthermore, with in situ hybridization, packaged transcripts were observed throughout the cytoplasm of the infected cells, including the site of virus assembly. Several proteins that nonspecifically interact with RNA, including the tegument protein pp28, were found within HCMV virions. These studies...Continue Reading

References

Jan 1, 1990·Current Topics in Microbiology and Immunology·M S CheeJ A Martignetti
Sep 1, 1993·Journal of Virology·W D Rawlinson, B G Barrell
Sep 1, 1996·Journal of Virology·C J Baldick, T Shenk
Jul 1, 1995·The Journal of General Virology·G HenselH F Kern
Jan 1, 1996·Intervirology·W Gibson
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·G A Smith, L W Enquist
Apr 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·D MuriauxA Rein
Jan 19, 2002·Journal of Virology·Thomas C Mettenleiter
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 28, 2002·Journal of Virology·Barbara G KluppThomas C Mettenleiter
Jun 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Maria Teresa SciortinoBernard Roizman

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Citations

Nov 27, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jörg Schröer, Thomas Shenk
Aug 28, 2009·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Emily E Marshall, Adam P Geballe
Aug 5, 2011·Journal of Virology·Ryan S NoyceKaren L Mossman
Jan 5, 2007·Journal of Virology·Scott TerhuneDong Yu
Dec 26, 2008·Journal of Virology·Anna R CliffeBernadette M Dutia
Jul 30, 2005·Journal of Virology·Jill BechtelDon Ganem
Oct 18, 2005·Journal of Virology·Adewunmi A Onafuwa-NugaAlice Telesnitsky
Jun 7, 2008·Microbiology and Molecular Biology Reviews : MMBR·Robert F Kalejta
Feb 7, 2009·Virology Journal·Li Xing, Suresh K Tikoo
Feb 11, 2014·Viruses·Rebecca Marie SmithJulie Anne Kerry
Jul 26, 2013·Archives of Virology·Cui-Qing YangMin-Hua Luo
Feb 3, 2005·Proceedings of the National Academy of Sciences of the United States of America·Maria C SilvaThomas Shenk
Nov 13, 2004·Proceedings of the National Academy of Sciences of the United States of America·Dai WangThomas Shenk
Dec 17, 2014·Proceedings of the National Academy of Sciences of the United States of America·Jesse HwangThomas Shenk
Aug 5, 2009·Virus Research·Thomas C MettenleiterHarald Granzow
Mar 16, 2012·Annals of the New York Academy of Sciences·Andrew J Davison
Apr 7, 2016·Virology Journal·Maria Kalamvoki, Thibaut Deschamps
Jan 4, 2006·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Richard AdairAnamaris M Colberg-Poley
Oct 12, 2016·Nature Communications·Sarah R Bordenstein, Seth R Bordenstein
Jun 8, 2017·The Journal of General Virology·Abdul-Aleem MohammadCecilia Söderberg-Nauclér
Dec 13, 2018·Frontiers in Microbiology·Philippe ColsonDidier Raoult
Aug 9, 2020·Frontiers in Microbiology·Yu-Qing Wang, Xiang-Yu Zhao
Aug 22, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Amelia K PintoAnn B Hill
Aug 24, 2018·Viruses·Christopher M GoodwinJoshua Munger

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