PMID: 6171808Oct 1, 1981Paper

RNAs involved in copy-number control and incompatibility of plasmid R1

Proceedings of the National Academy of Sciences of the United States of America
P StougaardK Nordström

Abstract

Replication of plasmid R1 is controlled by the products of two genes, copA and copB, that act as inhibitors of replication. Here it is shown that one small RNA synthesized from the copA gene acts as replication inhibitor. This RNA molecule was identified from analyses of RNAs synthesized in EScherichia coli minicells carrying R1 miniplasmids or chimeric plasmids containing the copA gene. In minicells, This RNA was found to be unstable with a half-life of less than a few minutes. Two mutant hybrid plasmids lacking the inhibitor function did not express the RNA normally made from plasmids carrying the wild-type copA allele. Nucleotide sequence analysis of one of the copA mutants showed that a base substitution had occurred within the promoter sequence in front of the copA gene. DNA sequence analysis of the other mutant showed that a putative transcription-termination sequence was affected. The DNA sequence analysis also showed that the RNA molecule synthesized from the copA gene is untranslatable but has the potential for a high degree of secondary structure.

References

Jan 1, 1979·Annual Review of Genetics·M Rosenberg, D Court
Jan 1, 1979·Cold Spring Harbor Symposia on Quantitative Biology·G HobomH Kössel
Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A M Maxam, W Gilbert
Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·C P GrayH Schaller
Mar 7, 1977·Molecular & General Genetics : MGG·G Dougan, D Sherratt
Apr 29, 1977·Molecular & General Genetics : MGG·J J Manis, B C Kline
Dec 15, 1977·Nature·N KennedyP Herrlich
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M De WildeF J Schmidt
Sep 25, 1978·Journal of Molecular Biology·J H CrosaS Falkow
Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M Inuzuka, D R Helinski
Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·K SugimotoY Hirota
Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M MeijerH Schaller
Jul 13, 1979·Molecular & General Genetics : MGG·P A Meacock, S N Cohen
Apr 23, 1976·Molecular & General Genetics : MGG·G O HumphreysE S Anderson
Jul 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·A FolkmanisH Echols
Apr 2, 1975·Biochimica Et Biophysica Acta·G O HumphreysE S Anderson
Dec 30, 1975·Molecular & General Genetics : MGG·K Matsubara, Y Takeda
Aug 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·S N CohenL Hsu
Apr 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·D B Clewell, D R Helinski
Jul 14, 1968·Journal of Molecular Biology·H Dürwald, H Hoffmann-Berling
May 27, 1967·Nature·E Meynell, N Datta
Jan 1, 1981·Molecular & General Genetics : MGG·P StougaardF G Hansen

❮ Previous
Next ❯

Citations

Nov 15, 2002·Biotechnology and Bioengineering·Stefan Schlatter, Martin Fussenegger
Jan 1, 1982·Molecular & General Genetics : MGG·J Light, S Molin
Jan 1, 1985·Molecular & General Genetics : MGG·J LightS Molin
Jan 1, 1983·Molecular & General Genetics : MGG·J Light, S Baumberg
Jan 1, 1989·Molecular & General Genetics : MGG·H YasuedaT Itoh
Jan 1, 1984·Molecular & General Genetics : MGG·V Shinger, C M Thomas
Jan 1, 1984·Molecular & General Genetics : MGG·B R BaumstarkJ R Scott
Oct 1, 1986·Molecular & General Genetics : MGG·K A ArmstrongE Ohtsubo
Jan 1, 1985·Folia Microbiologica·J Nesvera, J Hochmannová
Jun 21, 2013·Current Microbiology·Sung-Min KangHeon-Jin Lee
Jan 1, 1983·Bio Systems·L AlberghinaG Bortolan
Jun 6, 2002·Trends in Genetics : TIG·E Gerhart H Wagner, Klas Flärdh
Dec 30, 1998·Trends in Biochemical Sciences·E G Wagner, S Brantl
Jun 23, 2004·Nature Biotechnology·Farren J IsaacsJames J Collins
Apr 17, 2010·Nature Reviews. Genetics·Ahmad S Khalil, James J Collins
Dec 2, 2009·IET Systems Biology·F XuR Wang
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·D M StalkerD R Helinski
Nov 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·H MasaiK Arai
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·T MizunoM Inouye
Feb 1, 1986·Journal of Biomolecular Structure & Dynamics·V BrendelE N Trifonov
Apr 25, 1983·Nucleic Acids Research·D K Hawley, W R McClure
May 25, 1984·Nucleic Acids Research·V Brendel, E N Trifonov
Mar 25, 1986·Nucleic Acids Research·E GerhartK Nordström
Oct 24, 1986·Nucleic Acids Research·K Kim, R J Meyer
Feb 11, 1992·Nucleic Acids Research·S Brantl, D Behnke
Sep 15, 2005·Nucleic Acids Research·Amélie E CoudertFrédéric Lezot
Sep 12, 2012·Nucleic Acids Research·Dominik JägerRuth A Schmitz
Aug 24, 2007·PLoS Biology·Erel LevineTerence Hwa
Oct 28, 2008·Biological Chemistry·Stefano MarziThomas Geissmann
Feb 12, 2009·Future Microbiology·Sabine Brantl
Dec 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·G Ebersbach, K Gerdes
Aug 17, 2010·Annual Review of Genetics·Maureen Kiley Thomason, Gisela Storz
Sep 13, 2014·BMC Genomics·María Gómez-LozanoSøren Molin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.