PMID: 8970988Dec 1, 1996Paper

RNase H domain of Moloney murine leukemia virus reverse transcriptase retains activity but requires the polymerase domain for specificity

Journal of Virology
S J Schultz, J J Champoux

Abstract

The reverse transcriptase-associated RNase H activity of Moloney murine leukemia virus specifically cleaves within the polypurine tract region of the viral genome to generate the primer for plus-strand DNA synthesis and removes the tRNA primer after minus-strand initiation by preferentially cleaving the RNA one nucleotide before the RNA-DNA junction. Moreover, the enzyme is unable to cleave the extended tRNA substrate at the RNA-DNA junction even at high enzyme concentrations. The RNase H domain of the reverse transcriptase was expressed as a glutathione S-transferase fusion protein and purified from Escherichia coli extracts. Following removal of the glutathione S-transferase portion of the protein, the specificity of the isolated RNase H domain was determined in the plus-strand primer reaction and in the tRNA primer removal reaction. Although the isolated domain lacked specificity in both cases, it was still unable to cleave the tRNA substrate precisely at the RNA-DNA junction. Specificity in both cases could be restored by adding back a truncated form of Moloney murine leukemia virus reverse transcriptase lacking the RNase H domain. These results implicate the polymerase domain as a specificity determinant for the RNase H ac...Continue Reading

References

Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·V GopalakrishnanS J Benkovic
Jul 1, 1992·Journal of Virology·T B Fu, J Taylor
Jan 1, 1992·Analytical Biochemistry·W P Deng, J A Nickoloff
Feb 1, 1990·Journal of Virology·G X LuoJ Taylor
Feb 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·Z HostomskyB R Nodes
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·N Tanese, S P Goff
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·V R Prasad, S P Goff
Nov 1, 1984·Journal of Virology·J K SmithJ M Taylor
Oct 15, 1981·Nature·T M ShinnickJ G Sutcliffe
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·C ShoemakerD Baltimore
Feb 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·A Telesnitsky, S P Goff

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Citations

Mar 28, 1998·The Journal of Biological Chemistry·C PalaniappanR A Bambara
Jul 29, 2000·The Journal of Biological Chemistry·S J SchultzJ J Champoux
Feb 21, 2009·The FEBS Journal·James J Champoux, Sharon J Schultz
Jun 1, 2002·The Journal of Biological Chemistry·Mónica OlivaresManuel C López
Nov 27, 1999·The Journal of Biological Chemistry·S J SchultzJ J Champoux
Apr 2, 2010·Cellular and Molecular Life Sciences : CMLS·Alon Herschhorn, Amnon Hizi
Aug 3, 2019·DNA Repair·Malwina HyjekMarcin Nowotny
Feb 14, 2012·Retrovirology·Berit LeoBirgitta M Wöhrl
Feb 12, 2008·Virus Research·Sharon J Schultz, James J Champoux
Feb 4, 2010·Journal of Molecular Biology·Sharon J SchultzJames J Champoux

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