PMID: 9660177Jul 11, 1998Paper

RNase L inhibitor (RLI) antisense constructions block partially the down regulation of the 2-5A/RNase L pathway in encephalomyocarditis-virus-(EMCV)-infected cells

European Journal of Biochemistry
C MartinandC Bisbal

Abstract

The interferon-(IFN)-inducible 2',5'-oligoadenylate (2-5A)/endoribonuclease L (RNase L) pathway plays a major role in the antiviral and antiproliferative effects of IFN. The 2-5A/RNase L pathway appears to be regulated by the cell-growth status or viral infection. Viruses, and picornaviruses in particular, have evolved strategies to escape the 2-5A/RNase L-pathway-associated antiviral activity. We have recently cloned a cDNA coding for RLI, a RNase-L-specific protein inhibitor. Its regulated expression by viral infection could provide a new strategy to modulate the 2-5A/RNase L pathway. Since RNase L had been shown to be down regulated upon encephalomyocarditis (EMCV) infection, we stably transfected HeLa cells with a RLI antisense cDNA expressing vector. Four independent clones named VAS1, VAS2, VAS3 and VAS4 and one clone transfected with the empty vector (VV) as control, were analyzed. The level of RLI was decreased by 20% for VAS1, 25% for VAS2, 75% for VAS3 and 50% for VAS4. The inactivation of RNase L observed during EMCV infection was decreased in these clones as compared to control HeLa cells. Here again the results vary between the four clones. The maximum inhibition of RNase L (90%) was observed in control cells and i...Continue Reading

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Citations

Apr 28, 2001·Cytokine & Growth Factor Reviews·D E Levy, A García-Sastre
Aug 5, 2000·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·F Le RoyC Bisbal
Jan 7, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·C MartinandC Bisbal
Jan 27, 2005·Viral Immunology·Friedemann WeberOtto Haller
Dec 21, 2012·Journal of Virology·Stéphanie CordeilAndrea Cimarelli
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Aug 2, 2015·Current Opinion in Virology·Melissa Drappier, Thomas Michiels
Jan 11, 2000·The American Journal of Medicine·K De MeirleirB Lebleu
Aug 9, 2007·Cytokine & Growth Factor Reviews·Otto HallerFriedemann Weber
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Nov 12, 2013·Virus Research·Damien VitourStéphan Zientara
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Jan 7, 2021·MBio·Daniel Gonçalves-Carneiro, Paul D Bieniasz

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