RNF12 is an X-Encoded dose-dependent activator of X chromosome inactivation

Cell
Iris JonkersJoost Gribnau

Abstract

In somatic cells of female placental mammals, one X chromosome is inactivated to minimize sex-related dosage differences of X-encoded genes. Random X chromosome inactivation (XCI) in the embryo is a stochastic process, in which each X has an independent probability to initiate XCI, triggered by the nuclear concentration of one or more X-encoded XCI-activators. Here, we identify the E3 ubiquitin ligase RNF12 as an important XCI-activator. Additional copies of mouse Rnf12 or human RNF12 result in initiation of XCI in male mouse ES cells and on both X chromosomes in a substantial percentage of female mouse ES cells. This activity is dependent on an intact open reading frame of Rnf12 and correlates with the transgenic expression level of RNF12. Initiation of XCI is markedly reduced in differentiating female heterozygous Rnf12(+/-) ES cells. These findings provide evidence for a dose-dependent role of RNF12 in the XCI counting and initiation process.

References

Mar 20, 1997·Nature·L B HerzingA Ashworth
Mar 20, 1998·Cell·Y MarahrensR Jaenisch
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·J T LeeY Han
Nov 20, 2001·Molecular and Cellular Biology·S LuikenhuisR Jaenisch
Aug 6, 2003·Cytogenetic and Genome Research·T SadoH Sasaki
Sep 4, 2003·Genes & Development·Tatyana B NesterovaNeil Brockdorff
Jan 24, 2004·Methods in Cell Science : an Official Journal of the Society for in Vitro Biology·Thomas BeckerIngolf Bach
May 2, 2006·Proceedings of the National Academy of Sciences of the United States of America·Sébastien VigneauPhilippe Clerc
Nov 17, 2006·Development·Takashi SadoHiroyuki Sasaki
Jan 16, 2007·Molecular Cell·Mary E DonohoeJeannie T Lee
Sep 18, 2007·Current Opinion in Genetics & Development·Anton Wutz, Joost Gribnau
Jul 16, 2008·Molecular and Cellular Biology·Iris JonkersJoost Gribnau
Sep 20, 2008·Science·Pablo NavarroPhilip Avner
Jan 24, 2009·The Journal of Biological Chemistry·Yoon Ra Her, In Kwon Chung
Mar 11, 2009·Proceedings of the National Academy of Sciences of the United States of America·Catherine PatratEdith Heard

❮ Previous
Next ❯

Citations

Oct 12, 2012·Cellular and Molecular Life Sciences : CMLS·Tatsuya Ohhata, Anton Wutz
May 10, 2011·Human Genetics·Christine YangCarolyn J Brown
May 14, 2011·Human Genetics·Susanne ArtholdAnton Wutz
Jun 1, 2011·Human Genetics·Daphne B Pontier, Joost Gribnau
Dec 4, 2013·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Emily MaclarySundeep Kalantry
Mar 18, 2011·Journal of Human Genetics·Nisa K E RenaultWenda L Greer
Nov 24, 2011·Nature Reviews. Molecular Cell Biology·Jeannie T Lee
Dec 30, 2011·Stem Cells and Development·Valentina T StefanovaChristoph Hansis
Feb 9, 2012·Stem Cells and Development·Pablo Bermejo-AlvarezAlfonso Gutierrez-Adan
Jul 9, 2011·Nucleic Acids Research·Tahsin Stefan BarakatJoost Gribnau
Nov 21, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Takashi Sado, Neil Brockdorff
Oct 15, 2010·Cold Spring Harbor Perspectives in Biology·Jennifer C Chow, Edith Heard
Mar 31, 2011·Cold Spring Harbor Symposia on Quantitative Biology·L L Hall, J B Lawrence
Nov 3, 2011·PLoS Computational Biology·Antonio ScialdoneMario Nicodemi
Feb 8, 2011·PLoS Genetics·Tahsin Stefan BarakatJoost Gribnau
Feb 8, 2011·PLoS Genetics·Edda G SchulzEdith Heard
May 19, 2012·PloS One·Konstantin E OrishchenkoSuren M Zakian
Jun 10, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·Rabindranath De La FuenteMaria M Viveiros
Aug 2, 2011·Annual Review of Cell and Developmental Biology·Jane Lynda Deuve, Philip Avner
Jun 8, 2011·Human Genetics·I M van den BergJ H van Doorninck
Jan 9, 2014·European Journal of Human Genetics : EJHG·Erwin BrosensAnnelies de Klein
Jun 21, 2011·Human Genetics·Martin Escamilla-Del-ArenalEdith Heard
May 21, 2014·ELife·Tomasz ChelmickiAsifa Akhtar
Sep 12, 2014·Nature Communications·Mélanie MakhloufClaire Rougeulle
Sep 15, 2012·Annual Review of Genetics·Christine M Disteche
Jul 9, 2014·Annual Review of Cell and Developmental Biology·Anne-Valerie Gendrel, Edith Heard
Oct 19, 2010·Biologie aujourd'hui·Elphège P Nora, Edith Heard
Sep 17, 2010·Expert Review of Molecular Diagnostics·Jacques C Giltay, Merel C Maiburg
Jul 23, 2015·Chromosoma·Joke G van BemmelJoost Gribnau
Jul 15, 2015·Critical Reviews in Biochemistry and Molecular Biology·Shabnam MassahGratien G Prefontaine
Jan 8, 2016·Proceedings of the National Academy of Sciences of the United States of America·Srimonta GayenSundeep Kalantry
Jan 23, 2016·Trends in Biochemical Sciences·Cheryl MaduroJoost Gribnau
Aug 19, 2015·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Thomas Dixon-McDougall, Carolyn Brown
Mar 20, 2012·Current Opinion in Genetics & Development·Yesu JeonJeannie T Lee

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Cell eTOC

Cell is a scientific journal publishing research across a broad range of disciplines within the life sciences field. Discover the latest research from Cell here.