Rnf2 knockdown reduces cell viability and promotes cell cycle arrest in gastric cancer cells

Oncology Letters
Jingfang ZhangJisheng Zhang

Abstract

Rnf2 is a fundamental component of the polycomb repressive complex 1and acts as the really interesting new gene finger E3 ligase, which is responsible for histone 2A modification. Previous studies have shown that the ring finger protein 2 (Rnf2) is overexpressed in various types of tumor and has a close association with tumor development. However, few studies have been carried out into the expression and biological function of Rnf2 in gastric cancer cells. The present study measured the expression of Rnf2 in gastric cancer cells and normal epithelial gastric cells. The results demonstrate that Rnf2 is upregulated in gastric cancer cells. In addition, the knockdown of Rnf2 inhibited the cell viability and induced increased G1 phase followed by a substantial reduction of the G2/M phase. The expression levels of p21 and p27 were also significantly elevated by the knockdown of Rnf2. These results provide evidence of the oncogenic function of Rnf2 in gastric cancer, possibly through an inhibition of cellular proliferation and a delay of the G2/M phase. Therefore, Rnf2 may be a novel target for the prognosis and therapy of gastric cancer.

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Citations

Jul 25, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Meng LiDeng Chen
Jan 24, 2019·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Jie YangShuchai Zhu
May 23, 2019·Molecular Medicine Reports·Yü LiuQun Zhao
Jan 5, 2021·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Huizhen WangYongxiang Li
Jan 6, 2021·Journal of Cellular Physiology·Qi YanJian Du
Jan 16, 2021·Digestive Diseases and Sciences·Furong YueJun Zhang
Apr 6, 2021·Frontiers in Cell and Developmental Biology·Qi YanJian Du
Jul 6, 2021·The Journal of Biological Chemistry·Naga Padma Lakshmi ChSundarasamy Mahalingam

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