RNG1 is a late marker of the apical polar ring in Toxoplasma gondii.

Cytoskeleton
Johnson Q TranNaomi S Morrissette

Abstract

The asexually proliferating stages of apicomplexan parasites cause acute symptoms of diseases such as malaria, cryptosporidiosis and toxoplasmosis. These stages are characterized by the presence of two independent microtubule organizing centers (MTOCs). Centrioles are found at the poles of the intranuclear spindle. The apical polar ring (APR), a MTOC unique to apicomplexans, organizes subpellicular microtubules which impose cell shape and apical polarity on these protozoa. Here we describe the characteristics of a novel protein that localizes to the APR of Toxoplasma gondii which we have named ring-1 (RNG1). There are related RNG1 proteins in Neospora caninum and Sarcocystis neurona but no obvious homologs in Plasmodium spp., Cryptosporidium spp. or Babesia spp. RNG1 is a small, low-complexity, detergent-insoluble protein that assembles at the APR very late in the process of daughter parasite replication. We were unable to knock-out the RNG1 gene, suggesting that its gene product is essential. Tagged RNG1 lines have also allowed us to visualize the APR during growth of Toxoplasma in the microtubule-disrupting drug oryzalin. Oryzalin inhibits nuclear division and cytokinesis although Toxoplasma growth continues, and similar to e...Continue Reading

References

Jan 1, 1988·Archives of Disease in Childhood·M I Levene
May 1, 1987·The Journal of protozoology·B A Nichols, M L Chiappino
Aug 1, 1966·The Journal of Cell Biology·P K HeplerH Sprinz
Jan 1, 1994·Methods in Cell Biology·D S RoosA L Moulton
Mar 1, 1993·Nature Genetics·W Gish, D J States
Mar 1, 1996·The Journal of Eukaryotic Microbiology·R Mondragon, E Frixione
Jan 1, 1996·Methods in Enzymology·D G HigginsT J Gibson
Jan 1, 1994·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·D J States, W Gish
Dec 1, 1996·Experimental Parasitology·T J StokkermansD S Roos
Aug 8, 1997·Journal of Molecular Biology·M B Heintzelman, J D Schwartzman
Sep 7, 2000·Microbiology and Molecular Biology Reviews : MMBR·M W Black, J C Boothroyd
Jan 3, 2001·The Journal of Cell Biology·B StriepenD S Roos
Jun 23, 2001·Molecular and Biochemical Parasitology·J R RadkeM W White
Feb 7, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jason R SwedlowJohn M Murray
Feb 21, 2002·Molecular Biology of the Cell·Ke HuJohn M Murray
Mar 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Naomi S Morrissette, L David Sibley
Mar 20, 2002·The Journal of Cell Biology·Ke HuJohn M Murray
May 1, 2002·The EMBO Journal·Angelika Herm-GötzDominique Soldati
Aug 2, 2002·Nature·Laurence PelletierGraham Warren
Aug 15, 2002·The Journal of Biological Chemistry·Tara MannCon Beckers
Apr 23, 2003·Molecular and Biochemical Parasitology·Karl-Ferdinand Lechtreck
Mar 24, 2004·Current Opinion in Cell Biology·Dominique Soldati, Markus Meissner
May 5, 2004·The Journal of Cell Biology·Elizabeth GaskinsCon Beckers
Nov 19, 2004·International Review of Cytology·Ralph GräfIrene Schulz
Feb 22, 2005·International Journal for Parasitology·Ariela O KarasovGustavo Arrizabalaga
Nov 4, 2005·Molecular and Biochemical Parasitology·Jan HartmannGraham Warren
Feb 14, 2006·Journal of Muscle Research and Cell Motility·Angelika Herm-GötzDominique Soldati
May 11, 2006·Journal of Cell Science·Marc-Jan GubbelsBoris Striepen
Oct 14, 2006·Journal of Cell Science·Christiane Wiese, Yixian Zheng
Nov 7, 2006·Eukaryotic Cell·Luke A GilbertPeter J Bradley
Jan 25, 2007·Nature Reviews. Molecular Cell Biology·Jens Lüders, Tim Stearns
Nov 13, 2007·Nature Methods·Angelika Herm-GötzMarkus Meissner
Mar 4, 2008·Eukaryotic Cell·David J P FergusonMarc-Jan Gubbels
Mar 25, 2008·Molecular Biology and Evolution·Sven B GouldRoss F Waller
Apr 16, 2008·Journal of Cell Science·Manami NishiDavid S Roos
Aug 16, 2008·International Journal for Parasitology·Marc-Jan GubbelsTomasz Szatanek
Feb 17, 2009·Eukaryotic Cell·My-Hang Huynh, Vern B Carruthers
May 7, 2009·Cellular Microbiology·M González Del CarmenR Mondragón

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Citations

May 21, 2013·Eukaryotic Cell·Jessica Cruz de LeonNaomi S Morrissette
Oct 25, 2011·Eukaryotic Cell·Johnson Q TranNaomi S Morrissette
Jan 5, 2014·Nature Reviews. Microbiology·Maria E Francia, Boris Striepen
Jun 11, 2011·Trends in Parasitology·Jessica S TylerJohn C Boothroyd
Mar 25, 2018·Cytoskeleton·Harald PreisnerSven B Gould
Jan 14, 2016·PLoS Pathogens·Arnault GraindorgeDominique Soldati-Favre
Jul 7, 2020·PLoS Pathogens·Sarah K WilsonLaura J Knoll
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May 18, 2019·Nature Communications·Rachel M RudlaffJeffrey D Dvorin
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