Apr 20, 2016

Robust cell tracking in epithelial tissues through identification of maximum common subgraphs

BioRxiv : the Preprint Server for Biology
Jochen KursaweAlexander G Fletcher

Abstract

Tracking of cells in live-imaging microscopy videos of epithelial sheets is a powerful tool for investigating fundamental processes in embryonic development. Characterising cell growth, proliferation, intercalation and apoptosis in epithelia helps us to understand how morphogenetic processes such as tissue invagination and extension are locally regulated and controlled. Accurate cell tracking requires correctly resolving cells entering or leaving the field of view between frames, cell neighbour exchanges, cell removals and cell divisions. However, current tracking methods for epithelial sheets are not robust to large morphogenetic deformations and require significant manual interventions. Here, we present a novel algorithm for epithelial cell tracking, exploiting the graph-theoretic concept of a `maximum common subgraph' to track cells between frames of a video. Our algorithm does not require the adjustment of tissue-specific parameters, and scales in sub-quadratic time with tissue size. It does not rely on precise positional information, permitting large cell movements between frames and enabling tracking in datasets acquired at low temporal resolution due to experimental constraints such as photoxicity. To demonstrate the met...Continue Reading

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Mentioned in this Paper

Embryo
Cell Movement
Size
Cell Motility
Visual Tracking
Cell Division
Epithelium
Drosophila
Embryonic Development
Cell Proliferation

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