PMID: 3754394Feb 1, 1986Paper

Robust estimation of standard curves for protein molecular weight and linear-duplex DNA base-pair number after gel electrophoresis

Analytical Biochemistry
B D PlikaytisL W Mayer

Abstract

An accurate procedure for estimating linear-duplex DNA base-pair numbers and protein molecular weights after electrophoresis in single concentration gels is presented. A robust modified hyperbola was found to be superior for determining molecular weights and base-pair numbers for a set of known standards when compared with the conventional log transformation and a similar hyperbolic model. We describe the use of a soft laser-scanning densitometer to measure band-migration distances of wet, stained polyacrylamide gels for proteins and photographic negatives of agarose gels containing DNA stained with ethidium bromide. This automated densitometric method was more accurate than existing methods. A BASIC computer program detailing the procedure is included.

References

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Citations

Dec 1, 1986·Molecular & General Genetics : MGG·J E Pérez-OrtinL Franco
Jan 1, 1996·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·C GimenoJ García-de-Lomas
Sep 1, 1992·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M DasiJ Barberá
Jan 21, 1988·Journal of Immunological Methods·C M BlackC B Reimer
May 18, 1999·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·T R Janeiro-CinquiniC E Winter
Feb 1, 1995·Epidemiology and Infection·M BirchD W Denning
Feb 6, 1990·Biochemistry·A Marandici, C Monder
Jan 30, 2008·Current Protocols in Cell Biology·Scott Medberry, Sean Gallagher
Feb 12, 2008·Current Protocols in Molecular Biology·Scott MedberryButch Moomaw
Oct 28, 1994·Molecular & General Genetics : MGG·F A de FreitasA Leite
Apr 23, 2008·Current Protocols in Protein Science·Scott MedberryButch Moomaw
Mar 1, 1993·FEMS Microbiology Letters·E G BioscaC Amaro
Aug 1, 1992·Applied and Environmental Microbiology·M R Albiach, M M Lopez
Nov 1, 1987·Journal of Clinical Microbiology·B B PlikaytisH W Wilkinson
Nov 1, 1988·Applied and Environmental Microbiology·C AmaroE Alcaide
Aug 1, 1987·Journal of Clinical Microbiology·J A ElliottR R Facklam
Oct 1, 1994·Journal of Clinical Microbiology·B D PlikaytisG M Carlone

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