Robust high-throughput batch screening method in 384-well format with optical in-line resin quantification

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Jörg KittelmannJürgen Hubbuch

Abstract

High-throughput batch screening technologies have become an important tool in downstream process development. Although continuative miniaturization saves time and sample consumption, there is yet no screening process described in the 384-well microplate format. Several processes are established in the 96-well dimension to investigate protein-adsorbent interactions, utilizing between 6.8 and 50 μL resin per well. However, as sample consumption scales with resin volumes and throughput scales with experiments per microplate, they are limited in costs and saved time. In this work, a new method for in-well resin quantification by optical means, applicable in the 384-well format, and resin volumes as small as 0.1 μL is introduced. A HTS batch isotherm process is described, utilizing this new method in combination with optical sample volume quantification for screening of isotherm parameters in 384-well microplates. Results are qualified by confidence bounds determined by bootstrap analysis and a comprehensive Monte Carlo study of error propagation. This new approach opens the door to a variety of screening processes in the 384-well format on HTS stations, higher quality screening data and an increase in throughput.

References

Feb 28, 2003·Analytical Biochemistry·Stefan KreuschAnton Horn
Aug 20, 2004·Journal of Chromatography. a·Joachim ThiemannHartmut Schlüter
Jun 7, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·A Ralph Henderson
Jun 3, 2006·Biotechnology Progress·Tim HerrmannJürgen Hubbuch
Feb 22, 2007·Biotechnology and Applied Biochemistry·Marc D WengerDaniel G Bracewell
May 23, 2008·Biotechnology and Bioengineering·Jonathan L CoffmanBrian D Kelley
Jun 14, 2008·Biotechnology and Bioengineering·Brian D KelleyJon Coffman

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Citations

Jul 19, 2017·Microbial Cell Factories·P PhilipJ Büchs
Apr 26, 2020·Biotechnology Journal·Ana Cecília Afonso RoqueMarcel Ottens

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