Robust induction of neural crest cells to derive peripheral sensory neurons from human induced pluripotent stem cells.

Scientific Reports
Yoshie UmeharaKenji Takamori

Abstract

Because intractable itch reduces quality of life, understanding the fundamental mechanisms of itch is required to develop antipruritic treatments. Itch is mediated by peripheral sensory neurons, which originate from the neural crest (NC) during development. Itch-associated signaling molecules have been detected in genetically engineered animals and in cultures of peripheral neurons from dorsal root ganglia (DRG). Ethical difficulties collecting peripheral neurons from human DRG have limited analysis of itch in humans. This study describes a method of differentiating peripheral neurons from human induced pluripotent stem cells (hiPSCs) for physiological study of itch. This method resulted in the robust induction of p75 and HNK1 double-positive NC cells from hiPSCs. The expression of NC markers TFAP2A, SOX10 and SNAI1 increased during NC induction. The induction efficiency was nearly 90%, and human peripheral neurons expressing peripherin were efficiently differentiated from hiPSC-derived NC cells. Moreover, induced peripheral neurons expressed the sensory neuronal marker BRN3A and the itch-related receptors HRH1, MRGPRX1, IL31R and IL-4R. Calcium imaging analyses indicated that these peripheral neurons included sensory neurons r...Continue Reading

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Citations

Aug 8, 2020·Frontiers in Microbiology·Yimin WangYuan Sun
Dec 3, 2020·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Zhiqiang GaoYunxia Wu
Jun 16, 2021·Brain : a Journal of Neurology·Steven J MiddletonDavid L Bennett

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Methods Mentioned

BETA
fluorescence activated cell sorting
FACS
Flow cytometry
Fluorescence
PCR

Software Mentioned

CellQuest
MetaFluor

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