Oct 30, 2018

Robust, reproducible, and economical phosphopeptide enrichment using calcium titanate

BioRxiv : the Preprint Server for Biology
Adnan AhmedNoah Dephoure

Abstract

Mass spectrometry based phosphoproteomics has revolutionized phosphoprotein analysis and enhanced our understanding of diverse and fundamental cellular processes important for human health. Because of their relative scarcity, phosphopeptides must be enriched before analysis. We demonstrate an effective and robust single-step enrichment method using an off-the-shelf preparation of calcium titanate. Our method achieves a purity and depth of analysis comparable to a widely used TiO2 based method at a reduced cost and effort.

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Mentioned in this Paper

Calcium [EPC]
Laboratory Procedures
Exertion
Cellular Process
Calcium
Rutile
Mass Spectrometry
Phosphopeptides
Analysis
Calcium Supplements

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