DOI: 10.1101/457275Oct 30, 2018Paper

Robust, reproducible, and economical phosphopeptide enrichment using calcium titanate

BioRxiv : the Preprint Server for Biology
Adnan AhmedNoah Dephoure


Mass spectrometry based phosphoproteomics has revolutionized phosphoprotein analysis and enhanced our understanding of diverse and fundamental cellular processes important for human health. Because of their relative scarcity, phosphopeptides must be enriched before analysis. We demonstrate an effective and robust single-step enrichment method using an off-the-shelf preparation of calcium titanate. Our method achieves a purity and depth of analysis comparable to a widely used TiO2 based method at a reduced cost and effort.

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