Robust simple sequence repeat markers for spruce (Picea spp.) from expressed sequence tags

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Dainis RungisKermit Ritland

Abstract

Traditionally, simple sequence repeat (SSR)markers have been developed from libraries of genomic DNA. However, the large, repetitive nature of conifer genomes makes development of robust, single-copy SSR markers from genomic DNA difficult. Expressed sequence tags (ESTs), or sequences of messenger RNA, offer the opportunity to exploit single, low-copy, conserved sequence motifs for SSR development. From a 20,275-unigene spruce EST set, we identified 44 candidate EST-SSR markers. Of these, 25 amplified and were polymorphic in white, Sitka, and black spruce; 20 amplified in all 3 spruce species tested; the remaining five amplified in all except one species. In addition, 101 previously described spruce SSRs (mostly developed from genomic DNA), were tested. Of these, 17 amplified across white,Sitka, and black spruce. The 25 EST-SSRs had approximately 9% less heterozygosity than the 17 genomic-derived SSRs (mean H=0.65 vs 0.72), but appeared to have less null alleles, as evidenced by much lower apparent inbreeding (mean F=0.046 vs 0.126). These robust SSRs are of particular use in comparative studies,and as the EST-SSRs are within the expressed portion of the genome, they are more likely to be associated with a particular gene of int...Continue Reading

References

Mar 31, 1994·Nature·A M BowcockL L Cavalli-Sforza
Jan 1, 1995·Genetics·D B GoldsteinM W Feldman
Sep 27, 1994·Proceedings of the National Academy of Sciences of the United States of America·M B SoaresA Efstratiadis
Dec 1, 1993·Current Opinion in Genetics & Development·M W Bruford, R K Wayne
Aug 1, 1997·Genome Génome / Conseil National De Recherches Canada·A PfeifferM Morgante
Oct 6, 1999·Genome Research·X Huang, A Madan
Mar 4, 2000·Genome Génome / Conseil National De Recherches Canada·I ScottiP E Hedley
Jan 19, 2002·Nature Genetics·Michele MorganteWayne Powell
Feb 22, 2002·Current Opinion in Plant Biology·Antoni Rafalski
Jul 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·Y VigourouxJ Doebley
Feb 13, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·I. ScottiM. Morgante
Feb 13, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·I ScottiM Morgante
May 21, 2003·Molecular Ecology·J SquirrellW Powell
Jul 2, 2003·Genome Génome / Conseil National De Recherches Canada·Yanik BérubéKermit Ritland
Apr 10, 2004·The Journal of Heredity·B D Neff
Aug 1, 1993·Trends in Ecology & Evolution·D C QuellerC R Hughes
Jan 1, 2002·American Journal of Botany·Claire ArnoldRobert J Henry
Sep 1, 1996·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·W T van de Ven, R J McNicol

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Citations

Oct 11, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Betty PelgasNathalie Isabel
Nov 23, 2006·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Ramesh K AggarwalLalji Singh
Sep 23, 2008·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·I Fernandez-SilvaAntonio José Monforte
Apr 10, 2009·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Sylvie CloutierScott Duguid
May 24, 2007·Heredity·J R Ellis, J M Burke
Dec 7, 2007·Genome Génome / Conseil National De Recherches Canada·Camilo E LópezValérie Verdier
Feb 24, 2009·Genome Génome / Conseil National De Recherches Canada·K M OliveiraA P Souza
Aug 19, 2011·Genome Génome / Conseil National De Recherches Canada·B PranaviV Dinesh Kumar
Jun 19, 2007·BMC Genomics·Charles F Crane
Feb 15, 2013·Plant Methods·Péter PoczaiJaakko Hyvönen
Oct 14, 2011·BMC Research Notes·Silvia FluchGiovanni G Vendramin
Jun 19, 2008·Journal of Genetics·R YasodhaM Ghosh
May 2, 2012·Journal of Genetics·Moumita DasPushpendra K Gupta
Aug 22, 2014·BMC Plant Biology·Sonali Sachin RanadeMaría del Rosario García-Gil
Dec 4, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yue-Mei ZhaoGui-Fang Zhao
Feb 5, 2013·Molecular Ecology Resources·Ryo O GotohNaoto Hanzawa
Aug 28, 2015·Applications in Plant Sciences·Benjamin J MerrittJessica Brzyski
Jan 5, 2005·Trends in Biotechnology·Rajeev K VarshneyMark E Sorrells
Feb 14, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sima TaheriRedmond Ramin Shamshiri
May 31, 2011·American Journal of Botany·Andreas HomolkaSilvia Fluch

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