Robust transformation procedure for the production of transgenic farmer-preferred cassava landraces

Plant Methods
Ima M ZainuddinHervé Vanderschuren

Abstract

Recent progress in cassava transformation has allowed the robust production of transgenic cassava even under suboptimal plant tissue culture conditions. The transformation protocol has so far been used mostly for the cassava model cultivar 60444 because of its good regeneration capacity of embryogenic tissues. However, for deployment and adoption of transgenic cassava in the field it is important to develop robust transformation methods for farmer- and industry-preferred landraces and cultivars. Because dynamics of multiplication and regeneration of embryogenic tissues differ between cassava genotypes, it was necessary to adapt the efficient cv. 60444 transformation protocol to genotypes that are more recalcitrant to transformation. Here we demonstrate that an improved cassava transformation protocol for cv. 60444 could be successfully modified for production of transgenic farmer-preferred cassava landraces. The modified transformation method reports on procedures for optimization and is likely transferable to other cassava genotypes reportedly recalcitrant to transformation provided production of high quality FEC. Because the three farmer-preferred cassava landraces selected in this study have been identified as resistant or t...Continue Reading

References

Jan 5, 2005·Plant Molecular Biology·Hernán CeballosAlfred G O Dixon
Feb 2, 2008·Science·David B LobellRosamond L Naylor
Mar 21, 2009·Plant Molecular Biology·Hervé VanderschurenWilhelm Gruissem
Jan 8, 2011·Plant Cell Reports·Simon E BullHervé Vanderschuren
Apr 30, 2011·Annual Review of Plant Biology·Richard SayrePeng Zhang
May 14, 2011·Journal of Integrative Plant Biology·Jia LiuPeng Zhang

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Citations

Nov 8, 2016·In Vitro Cellular & Developmental Biology. Plant : Journal of the Tissue Culture Association·Paul Chavarriaga-AguirreJoe Tohme
Jun 25, 2017·Annual Review of Virology·Chrissie Rey, Hervé Vanderschuren
Jun 6, 2019·Methods and Protocols·Ezequiel Matías LentzHervé Vanderschuren
Jun 14, 2019·Frontiers in Plant Science·Alejandro BrandPaul Chavarriaga-Aguirre
Aug 30, 2018·Plant Methods·Ezequiel Matias LentzHervé Vanderschuren
Jun 16, 2019·International Journal of Molecular Sciences·Julia JansingLuisa Bortesi
Jan 25, 2017·The New Phytologist·Mark C WilsonRebecca S Bart
Jun 19, 2021·Frontiers in Plant Science·Wilfred ElegbaHervé Vanderschuren
Jul 10, 2021·Plant Molecular Biology·Ayaka UkeMasashi Ugaki

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Datasets Mentioned

BETA
AF234297

Methods Mentioned

BETA
transgenic

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