Robust, vaccine-induced CD8(+) T lymphocyte response against an out-of-frame epitope.

The Journal of Immunology : Official Journal of the American Association of Immunologists
Nicholas J ManessDavid I Watkins

Abstract

Rational vaccines designed to engender T cell responses require intimate knowledge of how epitopes are generated and presented. Recently, we vaccinated 8 Mamu-A*02(+) rhesus macaques with every SIV protein except Envelope (Env). Surprisingly, one of the strongest T cell responses engendered was against the Env protein, the Mamu-A*02-restricted epitope, Env(788-795)RY8. In this paper, we show that translation from an alternate reading frame of both the Rev-encoding DNA plasmid and the rAd5 vector engendered Env(788-795)RY8-specific CD8(+) T cells of greater magnitude than "normal" SIV infection. Our data demonstrate both that the pathway from vaccination to immune response is not well understood and that products of alternate reading frames may be rich and untapped sources of T cell epitopes.

References

Feb 18, 2004·Nature Medicine·Thomas C FriedrichDavid I Watkins
Apr 14, 2004·The Journal of Experimental Medicine·Sylvain CardinaudAndré Habel
Oct 8, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·John T LoffredoAlessandro Sette
Oct 29, 2004·PLoS Biology·Susan R SchwabNilabh Shastri
Jan 26, 2007·Journal of Virology·Thomas C FriedrichDavid I Watkins
Feb 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jonah B SachaDavid I Watkins
Jul 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Karin LoréRichard A Koup
Oct 24, 2007·The Journal of Experimental Medicine·Nicholas J ManessDavid I Watkins

❮ Previous
Next ❯

Citations

Mar 11, 2011·Cellular and Molecular Life Sciences : CMLS·Shelley R Starck, Nilabh Shastri
Jan 13, 2010·The Journal of Experimental Medicine·Anju BansalPaul A Goepfert
Jan 10, 2012·Cold Spring Harbor Perspectives in Medicine·Richard A Koup, Daniel C Douek
Feb 19, 2010·Journal of Virology·Mauricio A MartinsDavid I Watkins
May 19, 2011·PLoS Pathogens·Sylvain CardinaudArnaud Moris
Dec 21, 2010·Immunologic Research·Stephanie A FreelGeorgia D Tomaras
Jan 21, 2014·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Anne BetPaul A Goepfert
Sep 1, 2015·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Anju BansalPaul A Goepfert
Jun 21, 2016·Immunological Reviews·Shelley R Starck, Nilabh Shastri
Oct 5, 2018·Frontiers in Immunology·Redmond P SmythJulia C Kenyon
May 21, 2021·Nature·Jessica Sook Yuin HoIvan Marazzi

❮ Previous
Next ❯

Related Concepts

Related Feeds

B cell Activation

B cell activation is initiated by the ligation of the B cell receptor with antigen and ultimately results in the production of protective antibodies against potentially pathogenic invaders. Here is the latest research.