Abstract
Rational vaccines designed to engender T cell responses require intimate knowledge of how epitopes are generated and presented. Recently, we vaccinated 8 Mamu-A*02(+) rhesus macaques with every SIV protein except Envelope (Env). Surprisingly, one of the strongest T cell responses engendered was against the Env protein, the Mamu-A*02-restricted epitope, Env(788-795)RY8. In this paper, we show that translation from an alternate reading frame of both the Rev-encoding DNA plasmid and the rAd5 vector engendered Env(788-795)RY8-specific CD8(+) T cells of greater magnitude than "normal" SIV infection. Our data demonstrate both that the pathway from vaccination to immune response is not well understood and that products of alternate reading frames may be rich and untapped sources of T cell epitopes.
References
Oct 26, 1987·Nucleic Acids Research·M Kozak
Jan 1, 1995·Molecular and Cellular Biology·S PandeP J Farabaugh
Sep 1, 1994·Journal of Virology·P BorrowM B Oldstone
Jul 14, 1999·Immunity·S MalarkannanN Shastri
Nov 9, 2000·Journal of Virology·R IgnatiusM Pope
Sep 6, 2003·Science·Susan R SchwabNilabh Shastri
Feb 14, 2004·Journal of Virology·Thomas C FriedrichDavid I Watkins
Feb 18, 2004·Nature Medicine·Thomas C FriedrichDavid I Watkins
Apr 14, 2004·The Journal of Experimental Medicine·Sylvain CardinaudAndré Habel
Oct 8, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·John T LoffredoAlessandro Sette
Oct 29, 2004·PLoS Biology·Susan R SchwabNilabh Shastri
May 30, 2006·Journal of Virology·Nancy A WilsonDavid I Watkins
Jan 26, 2007·Journal of Virology·Thomas C FriedrichDavid I Watkins
Feb 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jonah B SachaDavid I Watkins
Jul 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Karin LoréRichard A Koup
Aug 19, 2007·Journal of Virology·Jonah B SachaDavid I Watkins
Oct 24, 2007·The Journal of Experimental Medicine·Nicholas J ManessDavid I Watkins
Nov 15, 2007·PloS One·John T LoffredoDavid I Watkins
Jul 4, 2008·Journal of Virology·Jonah B SachaDavid I Watkins
Oct 23, 2008·PloS One·Shelley R StarckNilabh Shastri
Nov 11, 2008·Nature·Jinyan LiuDan H Barouch
Nov 28, 2008·Journal of Virology·Yaoyu E WangTodd M Allen
May 1, 2009·Journal of Virology·Nancy A WilsonDavid I Watkins
Jul 17, 2009·Journal of Virology·Nicholas J ManessDavid I Watkins
Citations
Mar 11, 2011·Cellular and Molecular Life Sciences : CMLS·Shelley R Starck, Nilabh Shastri
Jan 13, 2010·The Journal of Experimental Medicine·Anju BansalPaul A Goepfert
Jan 10, 2012·Cold Spring Harbor Perspectives in Medicine·Richard A Koup, Daniel C Douek
May 13, 2011·Journal of Virology·Jun LiuGeorge F Gao
Aug 6, 2010·Journal of Virology·Jonah B SachaDavid I Watkins
Feb 19, 2010·Journal of Virology·Mauricio A MartinsDavid I Watkins
Jul 7, 2012·PloS One·Stephane ChampiatDouglas F Nixon
Aug 29, 2012·PloS One·Justin M GreeneDavid H O'Connor
May 19, 2011·PLoS Pathogens·Sylvain CardinaudArnaud Moris
Apr 14, 2017·Cell Host & Microbe·Ross A PollackYa-Chi Ho
Dec 15, 2017·Journal of Virology·Blake SchouestNicholas J Maness
Nov 26, 2010·Gene Therapy·J M LukeJ A Williams
Sep 30, 2014·Wiley Interdisciplinary Reviews. RNA·Pierre Murat, Judy Tellam
Dec 21, 2010·Immunologic Research·Stephanie A FreelGeorgia D Tomaras
Jan 21, 2014·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Anne BetPaul A Goepfert
Sep 1, 2015·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Anju BansalPaul A Goepfert
Jun 21, 2016·Immunological Reviews·Shelley R Starck, Nilabh Shastri
Oct 5, 2018·Frontiers in Immunology·Redmond P SmythJulia C Kenyon
May 21, 2021·Nature·Jessica Sook Yuin HoIvan Marazzi