Robustness of the Line Probe Assay for the Rapid Diagnosis and Characterization of Mutations in Extensively Drug-Resistant Tuberculosis

Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease
Marilyn M NinanJoy S Michael

Abstract

Introduction: Extensively drug-resistant tuberculosis (XDRTB) is a public health concern. We evaluated the diagnostic accuracy of Genotype® MTBDRsl for detection of resistance to fluoroquinolones (FQs) and second-line injectable drugs (SLIDs) and characterized mutations seen. Materials and Methods: MTBDRsl was carried out either directly on sputum samples or indirectly on culture isolates (n = 100) from known multidrug-resistant tuberculosis (MDRTB) patients from July 2015 to September 2017. Diagnostic accuracy for the detection of resistance to FQs and SLIDs was calculated in comparison with conventional culture-based drug susceptibility testing. Mutations at the gyrA and rrs loci, as well as discrepant phenotypic and genotypic results, were studied. A subset of isolates underwent pyrosequencing. Results: Out of 100 MDRTB samples/isolates tested, 59% were pre-XDRTB and 7% were XDRTB. The sensitivity and specificity for the detection of resistance to FQs were 96.6% [95% confidence interval (CI): 88.3-99.6] and 80% [95% CI: 64.4-90.9] and those for SLIDs were 70% [95% CI: 34.8-93.3] and 100% [95% CI: 95.9-100]. The most frequent mutations were the absence of wild type 3 with corresponding mutation 3c (20/66) at the gyrA loc...Continue Reading

References

Sep 24, 2008·Seminars in Respiratory and Critical Care Medicine·Madhukar Pai, Richard O'Brien
Oct 23, 2012·American Journal of Respiratory and Critical Care Medicine·Marinus BarnardRichard O'Brien
Feb 6, 2013·Antimicrobial Agents and Chemotherapy·Analise Z ReevesJames E Posey
Sep 17, 2013·Journal of Proteomics·Bhavnesh KumarDeepa Bisht
Jan 17, 2014·The International Journal of Tuberculosis and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·N TukvadzeR R Kempker
Nov 26, 2015·The Journal of Antimicrobial Chemotherapy·L RigoutsA Van Deun
Dec 22, 2015·The International Journal of Tuberculosis and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·J DomínguezUNKNOWN RESIST-TB networks
Apr 5, 2017·Tropical Medicine & International Health : TM & IH·Shirly SuzanaJoy S Michael

❮ Previous
Next ❯

Methods Mentioned

BETA
bronchoalveolar lavage
PCR

Software Mentioned

MTBDRsl
STATA SE

Related Concepts

Related Feeds

CRISPR Screens in Drug Resistance

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on the application of CRISPR-Cas system in high-throughput genome-wide screens to identify genes that may confer drug resistance.

Related Papers

The International Journal of Tuberculosis and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease
P W EiJ S Lee
The International Journal of Tuberculosis and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease
H M SaidM M Ehlers
© 2022 Meta ULC. All rights reserved