Robustness of virus removal by protein A chromatography is independent of media lifetime

Journal of Chromatography. a
Scott LuteKurt Brorson

Abstract

The robustness of virus clearance with respect to protein A media reuse was demonstrated using media with four matrix chemistries: Protein A immobilized ProSep A, Poros A50, Protein A ceramic Hyper DF and MabSelect SuRe, an alkali resistant protein A ligand. Endogenous retrovirus clearance, step yield, impurity clearance and other performance parameters were evaluated periodically in media cycled up to 300 times. Media lifetime was generally limited by either declining step yield or media fouling. However, clearance of endogenous retrovirus remained in an acceptable range, either increasing or remaining constant. Multiply cycled media were tested for clearance of three viruses (SV40, X-MuLV, and MMV); clearance was comparable to naïve media. Overall, virus clearance by protein A chromatography appears to be extremely robust with respect to media age.

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Citations

Jan 24, 2014·BioMed Research International·Mayank SaraswatHarry Holthofer
Jul 19, 2013·Biotechnology and Bioengineering·Min ZhangQi Chen
Jan 18, 2011·Journal of Chromatography. a·Dariusch HekmatDirk Weuster-Botz
Aug 18, 2009·Biologicals : Journal of the International Association of Biological Standardization·Scott LuteKurt Brorson
Feb 26, 2013·Biotechnology and Bioengineering·Lisa Connell-CrowleyRon Gillespie
May 30, 2012·Biotechnology Progress·P A Marichal-Gallardo, M M Alvarez
Aug 13, 2011·Biotechnology and Bioengineering·Lisa Connell-CrowleyGanesh Vedantham
Oct 20, 2012·Biotechnology Journal·Anne Serdakowski LondonKasey Mackay
Jan 1, 2014·Journal of Separation Science·Abby J Schadock-Hewitt, R Kenneth Marcus
Jan 15, 2010·Biotechnology and Bioengineering·George MiesegaesKurt Brorson
Feb 17, 2015·Biotechnology Progress·Adith VenkiteshwaranDayue Chen
Oct 23, 2014·Biotechnology and Bioengineering·Julia Bach, Lisa Connell-Crowley
Jul 2, 2014·Biologicals : Journal of the International Association of Biological Standardization·Peter L Roberts
Jun 28, 2016·Journal of Chromatography. a·Deqiang YuZheng Jian Li
Jul 18, 2016·Journal of Chromatography. a·Mili Pathak, Anurag S Rathore
Jul 31, 2016·Biotechnology and Bioengineering·Sarah A JohnsonKurt A Brorson
Feb 22, 2017·Biotechnology Progress·Jennifer ZhangRob Gronke
May 1, 2018·Biotechnology Progress·Matthew R BrownDavid J Roush
Nov 20, 2018·Biotechnology and Bioengineering·Chinwei PanMin Zhang
May 8, 2019·Biotechnology and Bioengineering·James AngeloZhengjian Li
Jan 10, 2021·Biotechnology and Bioengineering·James M AngeloSanchayita Ghose

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