ROCK inhibitor enhances the growth and migration of BRAF-mutant skin melanoma cells

Cancer Science
Fei ChangXunwei Wu

Abstract

Rho-associated protein kinase (ROCK) plays crucial roles in the proliferation and migration of different types of cells. ROCK inhibitor Y-27632 was previously reported to inhibit melanoma cell growth, and ROCK signaling was suggested to be a therapeutic target for treating melanoma. However, the negative effect of Y-27632 on melanoma cells was mainly seen in studies on murine B16 melanoma cells. Here, we reported that ROCK inhibitor actually promoted human melanoma cell growth and migration in vitro. Y-27632 increased the growth and migration of BRAF-mutated melanoma cells but had a negative effect on wild-type melanoma cells or primary melanocytes. We discovered that Y-27632 enhanced the growth of BRAF-mutated melanoma cells through increased ATK and ERK activity. The in vivo study further confirmed the in vitro finding. These data suggested that the effect of ROCK inhibitor on melanoma cells is cell-context dependent, and the application of ROCK inhibitor in the treatment of melanoma requires further study.

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Citations

Apr 21, 2020·Frontiers in Cell and Developmental Biology·Natasha S Clayton, Anne J Ridley
Aug 10, 2020·Scientific Reports·Lucile PoissonDriffa Moussata
Jan 21, 2021·Cancer Metastasis Reviews·Carmen Ruggiero, Enzo Lalli
Dec 11, 2020·Laboratory Investigation; a Journal of Technical Methods and Pathology·Tingjian ZuGeorge F Murphy
Mar 9, 2021·PLoS Genetics·Arjun BhattacharyaMichael I Love
Mar 2, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Zhuqing Li, Kaitlin M Bratlie
Jul 30, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Graziella Ribeiro de SousaMaría Sol Brassesco
Aug 6, 2021·Frontiers in Cell and Developmental Biology·Shuangshuang WangXunwei Wu

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Methods Mentioned

BETA
FCS
ELISA
PCR
transfection

Software Mentioned

ImageJ

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