Rod outer segment maintenance is enhanced in the presence of bFGF, CNTF and GDNF

Experimental Eye Research
M E CarwileT W Kraft

Abstract

We employed a morphological assay of outer segment collapse to determine if growth factors or other supplements directly affect dissociated rod photoreceptors in vitro. The morphological changes in outer segments were correlated with the light responsiveness of rods. Time-lapse video microscopy was used to observe the collapse of rod outer segments from isolated single cells and small clumps of cells. A consistent pattern of outer segment collapse into the inner segment was observed, yielding a convenient assay of the effects of neurotrophic factors on photoreceptor functional maintenance. The functional state of rods, defined as light-responsiveness, was measured with suction electrode recordings and matched with the various stages of outer segment collapse. Ciliary neurotrophic factor (CNTF) and glial cell-line-derived neurotrophic factor (GDNF) at a high concentration, yielded statistically significant improvements in rat outer segment survival times. Basic fibroblast growth factor (bFGF), which rescues photoreceptors in several rodent models of retinal degeneration, produced a significant increase in survival time in the presence of the cofactor heparin. In 4 out of 10 cases using human tisue, bFGF also yielded a significan...Continue Reading

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Citations

Nov 27, 2008·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Gabriele ThumannSandra Johnen
Mar 17, 2010·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Sinisa D GrozdanicDonald S Sakaguchi
Dec 1, 2010·Journal of Ocular Biology, Diseases, and Informatics·Kamla DuttYang Cao
Apr 18, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Milam A BrantleyJeffrey Milbrandt
Jan 15, 2014·Survey of Ophthalmology·Anton M Kolomeyer, Marco A Zarbin
Sep 2, 2010·Experimental Eye Research·Pamela B YangHans S Keirstead
Apr 22, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Brian D PerkinsJohn E Dowling
Apr 16, 2015·Mediators of Inflammation·Maria H MadeiraAna R Santiago
Jul 20, 2014·Progress in Retinal and Eye Research·Nicolás CuencaIsabel Pinilla
Dec 6, 2014·Progress in Retinal and Eye Research·Marcus KarlstetterThomas Langmann
Dec 12, 2001·Molecular Therapy : the Journal of the American Society of Gene Therapy·L H McGee SanftnerJ G Flannery
Apr 5, 2007·Journal of Leukocyte Biology·Thomas Langmann
Jun 8, 2001·Progress in Retinal and Eye Research·R D LundJ M Lawrence
Jun 17, 2000·Progress in Lipid Research·N M GiustoM G Ilincheta de Boschero
Oct 20, 2020·Progress in Retinal and Eye Research·Christophe BaudouinElisabeth M Messmer

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