Rodent adrenocortical cells display high affinity binding sites and proteins for inhibin A, and express components required for autocrine signalling by activins and bone morphogenetic proteins

The Journal of Endocrinology
P G FarnworthJ K Findlay

Abstract

Inhibins are expressed in the adrenal cortex, but little is known of their binding or role in the adrenal. The aims of the present study were, first, to establish whether a mouse adrenocortical (AC) cell line expresses inhibins/activins and bone morphogenetic proteins (BMP), along with proteins required for inhibin to antagonise activin and BMP actions and, secondly, to characterise and compare inhibin binding sites and proteins in the rat adrenal gland and AC cells. AC cells were found to: (1) express mRNA for multiple BMPs (BMP-2, -3, -4, -6, -8a), growth/differentiation factors (GDF-1, -3, -5, -9), Lefty A and B, and the inhibin alpha, beta(A) and beta(B) subunits (2) secrete inhibin A and inhibin B and (3) express mRNA encoding the inhibin co-receptor, betaglycan, along with activin and BMP type I (ALK2-7) and type II (ActRII, ActRIIB, BMPRII) receptors, and binding proteins (follistatin, BAMBI, gremlin). When applied to sections of rat adrenal glands, [(125)I]inhibin A specifically bound to cells of the adrenal cortex, mainly in the zona reticularis. Scatchard analyses of in vitro [(125)I]inhibin A binding to dispersed rat adrenal cells and AC cells revealed sites of high affinity (K(d)(1) of 0.18 and 0.15 nM, respectively...Continue Reading

Citations

Jan 16, 2009·The Journal of Reproduction and Development·Kazuko KubotaTakashi Minegishi
Jun 15, 2007·Twin Research and Human Genetics : the Official Journal of the International Society for Twin Studies·Tiane ZhangZhiyong Yan
Sep 9, 2009·The Journal of Physiology·Buel D RodgersLuis F Santana
Apr 24, 2012·Molecular and Cellular Endocrinology·Maree Bilandzic, Kaye L Stenvers
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Dec 17, 2009·Trends in Endocrinology and Metabolism : TEM·Kaye L Stenvers, Jock K Findlay
Jan 5, 2011·Reproduction in Domestic Animals = Zuchthygiene·Fernanda Paulini, Eduardo O Melo
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Jul 29, 2016·Molecular and Cellular Endocrinology·Yao WangKaye L Stenvers
Aug 22, 2009·Biology of Reproduction·Mai A SarrajKaye L Stenvers

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