RodZ modulates geometric localization of the bacterial actin MreB to regulate cell shape

Nature Communications
Alexandre ColavinKerwyn Casey Huang

Abstract

In the rod-shaped bacterium Escherichia coli, the actin-like protein MreB localizes in a curvature-dependent manner and spatially coordinates cell-wall insertion to maintain cell shape, although the molecular mechanism by which cell width is regulated remains unknown. Here we demonstrate that the membrane protein RodZ regulates the biophysical properties of MreB and alters the spatial organization of E. coli cell-wall growth. The relative expression levels of MreB and RodZ change in a manner commensurate with variations in growth rate and cell width, and RodZ systematically alters the curvature-based localization of MreB and cell width in a concentration-dependent manner. We identify MreB mutants that alter the bending properties of MreB filaments in molecular dynamics simulations similar to RodZ binding, and show that these mutants rescue rod-like shape in the absence of RodZ alone or in combination with wild-type MreB. Thus, E. coli can control its shape and dimensions by differentially regulating RodZ and MreB to alter the patterning of cell-wall insertion, highlighting the rich regulatory landscape of cytoskeletal molecular biophysics.

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Citations

Jan 1, 2019·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Keisuke KuritaDaisuke Shiomi
Jan 10, 2020·AIMS Microbiology·Risa Ago, Daisuke Shiomi
Aug 1, 2020·Electrophoresis·Erin A Henslee
Jun 10, 2020·Proceedings of the National Academy of Sciences of the United States of America·Huan ZhangBeiyan Nan
Aug 26, 2020·Scientific Reports·Peter OatleyRobert P Fagan
Jul 20, 2018·Nature Communications·Benjamin P BrattonRandy M Morgenstein
Aug 29, 2019·ELife·Nikola OjkicShiladitya Banerjee
Feb 26, 2019·Microbial Biotechnology·Grégory BoëlAntoine Danchin
Mar 18, 2020·Nature Communications·Handuo ShiKerwyn Casey Huang
Dec 29, 2020·PLoS Genetics·Xiaolong LiuTanneke den Blaauwen
May 18, 2020·Biophysical Journal·John N WernerEric A Klein
Apr 28, 2021·Nature Chemical Biology·Edoardo RomanoBarbara Di Ventura
Jan 21, 2021·EcoSal Plus·Shambhavi GardeManjula Reddy
May 31, 2018·Chemical Reviews·David A DikShahriar Mobashery
Jun 30, 2021·Annual Review of Cell and Developmental Biology·Ethan C Garner

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Methods Mentioned

BETA
X-ray
dissection
PCR

Software Mentioned

NAMD
Morphometrics
VMD
MATLAB

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