Role and mechanism of microRNA-21 in H2O2-induced apoptosis in bone marrow mesenchymal stem cells

Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia
Chen LvGuanjun Tu

Abstract

microRNA-21 (miR-21) contributes to anti-apoptosis, proliferation and migration in many cells, but its role in inhibiting apoptosis in bone marrow mesenchymal stem cells (BMSC) remains unclear. The aim of this study was to determine the role of miR-21 in H2O2-induced BMSC apoptosis. We used quantitative real time-polymerase chain reaction (RT-PCR) to demonstrate the level of miR-21 after treatment of BMSC with H2O2. BMSC apoptosis was induced by different concentrations of H2O2 and was decreased in miR-21-upregulated cells. The expression of PTEN, a functional target gene of miR-21 in BMSC, was regulated by miR-21. The RT-PCR results indicated that miR-21 was significantly up-regulated, and western blot analysis indicated that Bcl-2 was up-regulated, whereas the apoptosis-related genes caspase 3/9 and Bax were down-regulated in miR-21-up-regulated cells. The miR-21-up-regulated cells had significantly enhanced Akt phosphorylation, as measured by western blot analysis. LY294002, an inhibitor of Akt activation, abolished the protective effects of miR-21-up-regulated cells. These results suggest that miR-21 contributes to inhibition of apoptosis in BMSC by down-regulating PTEN, potentially via the PI3K/Akt pathway.

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Citations

Jul 25, 2019·International Journal of Molecular Sciences·Jaime FreitasMaria Inês Almeida
Apr 14, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Zhen GengChangsheng Liu
Jan 12, 2021·Biochemistry and Biophysics Reports·Shunichi OkaUjjal K Bhawal
Jan 29, 2021·World Journal of Stem Cells·Alberto González-GonzálezFlor M Pérez-Campo

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