Role and structural characterization of plant aldehyde dehydrogenases from family 2 and family 7

The Biochemical Journal
Radka KončitíkováDavid Kopecný

Abstract

Aldehyde dehydrogenases (ALDHs) are responsible for oxidation of biogenic aldehyde intermediates as well as for cell detoxification of aldehydes generated during lipid peroxidation. So far, 13 ALDH families have been described in plants. In the present study, we provide a detailed biochemical characterization of plant ALDH2 and ALDH7 families by analysing maize and pea ALDH7 (ZmALDH7 and PsALDH7) and four maize cytosolic ALDH(cALDH)2 isoforms RF2C, RF2D, RF2E and RF2F [the first maize ALDH2 was discovered as a fertility restorer (RF2A)]. We report the crystal structures of ZmALDH7, RF2C and RF2F at high resolution. The ZmALDH7 structure shows that the three conserved residues Glu(120), Arg(300) and Thr(302) in the ALDH7 family are located in the substrate-binding site and are specific to this family. Our kinetic analysis demonstrates that α-aminoadipic semialdehyde, a lysine catabolism intermediate, is the preferred substrate for plant ALDH7. In contrast, aromatic aldehydes including benzaldehyde, anisaldehyde, cinnamaldehyde, coniferaldehyde and sinapaldehyde are the best substrates for cALDH2. In line with these results, the crystal structures of RF2C and RF2F reveal that their substrate-binding sites are similar and are form...Continue Reading

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Citations

Aug 12, 2015·Biochemistry·Min Luo, John J Tanner
Feb 22, 2017·Chemico-biological Interactions·Rosario A Muñoz-ClaresHéctor Riveros-Rosas
Jun 26, 2017·Journal of Plant Physiology·Shashank Sagar SainiDebabrata Sircar
Sep 6, 2018·FEBS Letters·David A KorasickJohn J Tanner
Aug 17, 2020·The Journal of Biological Chemistry·Soon Goo LeeJoseph M Jez
Jul 28, 2017·The Plant Journal : for Cell and Molecular Biology·Martina KopečnáDavid Kopečný
Jan 23, 2021·Plant Physiology and Biochemistry : PPB·Poonam SinghMariam Gaid

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