Role for arachidonic acid metabolism and protein synthesis in recombinant bovine interferon-gamma-induced activation of bovine neutrophils.

Journal of Leukocyte Biology
M J SteinbeckJ A Roth

Abstract

Bovine neutrophils were preincubated with recombinant bovine interferon-gamma (rboIFN-gamma), and the molecular events leading to enhanced antibody-dependent (ADCC) and antibody-independent (AINC) neutrophil-mediated cytotoxicity and reduced random migration under agarose were investigated. Addition of alpha-amanitin, puromycin, or cycloheximide (RNA and protein synthesis inhibitors) during preincubation and assaying prevented the rboIFN-gamma-induced AINC enhancement and migration inhibition, but did not influence the enhancement of ADCC. Treatment of neutrophils with rboIFN-gamma consistently increased the synthesis of a 60 and a 94-95 kilodalton protein and of leukotriene B4 and 5-HETE. Neutrophils isolated from dexamethasone-treated cattle showed similar protein synthetic activity after rboIFN-gamma treatment, but these cells failed to show enhanced AINC. Neutrophils from dexamethasone-treated cattle have been shown to have depressed arachidonic acid metabolism. Addition of lipoxygenase inhibitors (NDGA and BW755c) to neutrophils from nondexamethasone-treated cattle following preincubation with rboIFN-gamma (i.e., after protein synthesis had occurred) eliminated the enhancement of AINC. Thus it was concluded that the metabo...Continue Reading

Citations

May 16, 2001·Journal of Dairy Science·J A RothM Weighner
Apr 21, 2004·Immunology·Terri N Ellis, Blaine L Beaman
Jul 1, 1996·Critical Reviews in Oncology/hematology·C Denzlinger

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