PMID: 9420286Jan 7, 1998Paper

Role for calnexin and N-linked glycosylation in the assembly and secretion of hepatitis B virus middle envelope protein particles

Journal of Virology
M Werr, R Prange

Abstract

Unlike those of the S and the L envelope proteins, the functional role of the related M protein in the life cycle of the hepatitis B virus (HBV) is less understood. We now demonstrate that a single N glycan, specific for M, is required for efficient secretion of M empty envelope particles. Moreover, this glycan mediates specific association of M with the chaperone calnexin. Conversely, the N glycan, common to all three envelope proteins, is involved neither in calnexin binding nor in subviral particle release. As proper folding and trafficking of M need the assistance of the chaperone, the glycan-dependent association of M with calnexin may thus play a crucial role in the assembly of HBV. Beyond being modified by N glycosylation, M is modified by O glycosylation occurring within its amino acid sequence at positions 27 to 47. The O glycans, however, were found to be dispensable for secretion of M but may rather support viral infectivity. Surprisingly, nonglycosylated M localizes exclusively to the cytosol, either for degradation or for a yet-unknown function.

References

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Citations

May 23, 2002·Antiviral Chemistry & Chemotherapy·T M Block, R Jordan
Jul 18, 2002·Nature Reviews. Drug Discovery·Raymond A DwekNicole Zitzmann
Jun 26, 2004·The Journal of General Virology·Sigrid SchmittWolfram H Gerlich
Aug 14, 2009·Cellular Microbiology·Romuald PatientPhilippe Roingeard
Oct 15, 2013·Fundamental & Clinical Pharmacology·Li-Peng QiuKe-Ping Chen
Nov 1, 2013·Emerging Microbes & Infections·Jinhong ChangTimothy Block
Feb 7, 2002·Expert Opinion on Investigational Drugs·William DelaneyStephen A Locarnini
Feb 3, 2007·Journal of Virology·Romuald PatientPhilippe Roingeard
Feb 3, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yang LiuGong Cheng
Jun 10, 2020·Cells·Mihaela-Olivia DobricaNorica Branza-Nichita
Feb 24, 2001·Traffic·I Braakman, E van Anken
Sep 12, 2012·Medical Microbiology and Immunology·Reinhild Prange
May 1, 1999·Biological Chemistry·R PrangeH Löffler-Mary
Jul 27, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Pamela A NortonTimothy M Block
Nov 18, 2018·Frontiers in Microbiology·Fan Yang

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