Role for CysJ flavin reductase in molybdenum cofactor-dependent resistance of Escherichia coli to 6-N-hydroxylaminopurine.

Journal of Bacteriology
Stanislav G KozminRoel M Schaaper

Abstract

We have previously described a novel Escherichia coli detoxification system for the removal of toxic and mutagenic N-hydroxylated nucleobases and related compounds that requires the molybdenum cofactor. Two subpathways (ycbX and yiiM) were identified, each employing a novel molybdo activity capable of inactivating N-hydroxylated compounds by reduction to the corresponding amine. In the present study, we identify the cysJ gene product as one additional component of this system. While the CysJ protein has been identified as the NADPH:flavin oxidoreductase component of the CysJI sulfite reductase complex (CysJ(8)I(4)), we show that the role of CysJ in base analog detoxification is unique and independent of CysI and sulfite reductase. We further show that CysJ functions as a specific partner of the YcbX molybdoenzyme. We postulate that the function of CysJ in this pathway is to provide, via its NADPH:flavin reductase activity, the reducing equivalents needed for the detoxification reaction at the YcbX molybdocenter. In support of the proposed interaction of the CysJ and YcbX proteins, we show that an apparent CysJ-YcbX "hybrid" protein from two Vibrio species is capable of compensating for a double cysJ ycbX defect in E. coli.

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Citations

Feb 16, 2010·Journal of Bacteriology·Robert H White
Jan 29, 2014·Chemical Reviews·Russ HillePartha Basu
Sep 29, 2011·Drug Metabolism Reviews·Antje HavemeyerBernd Clement
Jul 31, 2013·MicrobiologyOpen·Stanislav G KozminRoel M Schaaper
Nov 27, 2014·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Gudrun OttBernd Clement
Mar 25, 2017·International Journal of Molecular Sciences·Alejandro Chamizo-AmpudiaAngel Llamas
May 13, 2017·BioFactors·Angel LlamasEmilio Fernandez
Jan 16, 2013·Dalton Transactions : an International Journal of Inorganic Chemistry·Russ Hille
Mar 7, 2018·Science Advances·Teruaki NakatsujiRichard L Gallo
May 31, 2011·Metallomics : Integrated Biometal Science·Angel LlamasAurora Galván
Jun 27, 2013·Metallomics : Integrated Biometal Science·Manuel Tejada-JiménezÁngel Llamas

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