Role for gene looping in intron-mediated enhancement of transcription.

Proceedings of the National Academy of Sciences of the United States of America
Aboudi M MoabbiAthar Ansari

Abstract

Intron-containing genes are often transcribed more efficiently than nonintronic genes. The effect of introns on transcription of genes is an evolutionarily conserved feature, being exhibited by such diverse organisms as yeast, plants, flies, and mammals. The mechanism of intron-mediated transcriptional activation, however, is not entirely clear. To address this issue, we inserted an intron in INO1, which is a nonintronic gene, and deleted the intron from ASC1, which contains a natural intron. We then compared transcription of INO1 and ASC1 genes in the presence and absence of an intron. Transcription of both genes was significantly stimulated by the intron. The introns have a direct role in enhancing transcription of INO1 and ASC1 because there was a marked increase in nascent transcripts from these genes in the presence of an intron. Intron-mediated enhancement of transcription required a splicing competent intron. Interestingly, both INO1 and ASC1 were in a looped configuration when their genes contained an intron. Intron-dependent gene looping involved a physical interaction of the promoter and the terminator regions. In addition, the promoter region interacted with the 5' splice site and the terminator with the 3' splice si...Continue Reading

References

Jun 25, 1992·Nucleic Acids Research·J J JonssonR S McIvor
Jun 1, 1991·Molecular and Cellular Biology·T ChoiR Jaenisch
Dec 1, 1987·Genes & Development·J CallisV Walbot
Jan 25, 1989·Nucleic Acids Research·T L DengL F Johnson
Oct 1, 1988·Molecular and Cellular Biology·A R Buchman, P Berg
Dec 1, 1994·Current Opinion in Genetics & Development·J S Mattick
Jan 26, 1996·Cell·G Dieci, A Sentenac
Aug 28, 1999·The Journal of Biological Chemistry·E J Cho, S Buratowski
Dec 2, 1999·Methods : a Companion to Methods in Enzymology·G Hager
Jan 11, 2000·Nucleic Acids Research·T H ChengM R Gartenberg
Oct 22, 2002·Nature Structural Biology·Kon Yew KwekAlexandre Akoulitchev
Nov 5, 2002·Genes & Development·Andre FurgerNick J Proudfoot
Apr 26, 2003·Trends in Biochemical Sciences·Hervé Le HirMelissa J Moore
Jul 21, 2004·Molecular Cell·José García-MartínezJosé E Pérez-Ortín
Dec 2, 2005·Genes & Development·Athar Ansari, Michael Hampsey
Jul 4, 2006·Genetics·Kara JuneauRonald W Davis
Jan 19, 2007·Biology of Reproduction·Martin CharronWilliam W Wright
Sep 7, 2007·Molecular Cell·Badri Nath Singh, Michael Hampsey
Jan 10, 2008·Molecular Microbiology·Patrizia Spitalny, Michael Thomm
Feb 5, 2008·Molecular Cell·Christian Kroun DamgaardJørgen Kjems
Feb 22, 2008·Molecular Biology of the Cell·Julie ParenteauSherif Abou Elela
Apr 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·Sue Mei Tan-WongMelissa A Brown
Jul 18, 2008·Current Topics in Microbiology and Immunology·A B Rose
Mar 10, 2009·Methods : a Companion to Methods in Enzymology·Badri Nath SinghMichael Hampsey
Jul 16, 2009·The Journal of Biological Chemistry·Belal El KaderiAthar Ansari
Nov 26, 2009·Genes & Development·Jean-Philippe LainéMichael Hampsey
Jan 30, 2010·Nature·Timothy W Nilsen, Brenton R Graveley
Mar 17, 2010·Current Biology : CB·Yuming WangStefan G E Roberts
Nov 13, 2010·Molecular Cell·Christophe K MapendanoTorben Heick Jensen
Feb 18, 2011·Cell Cycle·Søren Lykke-AndersenTorben Heick Jensen

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Citations

Oct 15, 2013·Molecular Biology and Evolution·Zhiming DaiXianhua Dai
Mar 12, 2013·The Journal of Biological Chemistry·Banupriya Mukundan, Athar Ansari
Jun 7, 2015·Journal of Dermatological Science·Yungling Leo LeeFu-Tong Liu
Feb 19, 2016·Cancer Biomarkers : Section a of Disease Markers·R FaryalM A Kayani
Jun 20, 2015·Plant Science : an International Journal of Experimental Plant Biology·Jenna E Gallegos, Alan B Rose
Apr 8, 2015·Nucleic Acids Research·Amandine BonnetBenoit Palancade
Apr 25, 2015·Nucleic Acids Research·Eleen Y ShumMiles F Wilkinson
Jan 29, 2014·Plant Science : an International Journal of Experimental Plant Biology·Carlos M Hernandez-Garcia, John J Finer
Aug 6, 2016·Plant Molecular Biology·Alan B RoseNoah Kojima
Oct 14, 2016·Applied Microbiology and Biotechnology·Hisashi HoshidaRinji Akada
Aug 20, 2015·Scientific Reports·Scott Medler, Athar Ansari
Dec 22, 2017·Tissue Engineering. Part B, Reviews·Ara Hacobian, David Hercher
Apr 17, 2018·The Plant Journal : for Cell and Molecular Biology·Qianli DongLei Gong
Jan 5, 2018·Current Genetics·Shravan Kumar Mishra, Poonam Thakran
Aug 10, 2017·Nature Reviews. Molecular Cell Biology·Lydia HerzelKarla M Neugebauer
Apr 3, 2014·The Journal of Biological Chemistry·Kamalpreet NagpalGeorge C Tsokos
Apr 3, 2016·The Journal of the Acoustical Society of America·David S MichaudFrits van den Berg
Apr 3, 2016·The Journal of the Acoustical Society of America·David S MichaudFrits van den Berg
Jul 28, 2020·Plants·David Zalabák, Yoshihisa Ikeda
Jul 22, 2017·Environmental Microbiology·Yanjun LiMingguo Zhou
Apr 23, 2015·Bioorganicheskaia khimiia·A P PereverzevN G Gurskaia
Mar 14, 2018·Proceedings of the National Academy of Sciences of the United States of America·Hisashi WadaIchiro Taniuchi
Jan 24, 2017·Frontiers in Plant Science·Miriam Laxa
Jul 16, 2019·Nucleic Acids Research·Paula Allepuz-FusterOlga Calvo

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