Role for myosin II in regulating positioning of Salmonella-containing vacuoles and intracellular replication.

Infection and Immunity
Julie A WasylnkaJohn H Brumell

Abstract

Salmonella enterica serovar Typhimurium grows within host cells in a permissive compartment termed the Salmonella-containing vacuole (SCV). These bacteria use two distinct type III secretion systems (T3SS) to deliver virulence proteins (effectors) into cells. Effectors secreted by the Salmonella pathogenicity island 1 (SPI-1)-encoded T3SS mediate invasion and early SCV maturation steps, while those secreted by the SPI-2 T3SS affect the SCV at later stages postinfection. Some SPI-2 effectors modulate microtubule motor activity on the SCV. Here, we show that the actin-based motor myosin II also affects SCV dynamics during infection. Following invasion, myosin II is required for SCV positioning near the nucleus of host cells. Later, myosin II counteracts the activities of the SPI-2 effectors PipB2 and SseJ to maintain SCV positioning and stability, respectively. Myosin II activity was required for maximal bacterial growth in macrophages. Rho kinase activity was required for SCV positioning. The effector SopB, a known activator of Rho GTPases, was found to be required for SCV positioning, and transfection of cells with SopB was sufficient to induce myosin II phosphorylation. These studies reveal a novel role for myosin II in contro...Continue Reading

References

Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J E Galán, R Curtiss
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P I FieldsF Heffron
Nov 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·F Garcia-del PortilloB B Finlay
Mar 19, 1996·Proceedings of the National Academy of Sciences of the United States of America·J E SheaD W Holden
Aug 23, 1996·The Journal of Biological Chemistry·M AmanoK Kaibuchi
Nov 20, 1998·The Journal of Clinical Investigation·J M KimM F Kagnoff
Nov 25, 1998·Proceedings of the National Academy of Sciences of the United States of America·F A NorrisP W Majerus
Nov 5, 1999·The Journal of Biological Chemistry·M AmanoK Kaibuchi
Jun 9, 2000·Molecular Microbiology·M Hensel
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·J E Galan, D Zhou
Sep 9, 2000·The Journal of Biological Chemistry·O Steele-MortimerB B Finlay
Apr 11, 2001·Molecular Biology of the Cell·J S BergR E Cheney
Feb 21, 2002·Molecular Biology of the Cell·Maria DiakonovaJoel A Swanson
Aug 27, 2002·Current Biology : CB·Isabel M OlazabalLaura M Machesky
Dec 31, 2002·American Journal of Physiology. Renal Physiology·Andras MassziAndrás Kapus
Oct 1, 2003·The EMBO Journal·Suzana P Salcedo, David W Holden
Feb 19, 2004·Journal of Cell Science·Julie GuignotDavid W Holden
May 4, 2004·Molecular Biology of the Cell·Rene E HarrisonSergio Grinstein
Jun 4, 2004·American Journal of Physiology. Cell Physiology·Lone S BertelsenKim E Barrett
Jun 19, 2004·Science·Lorraine D HernandezJorge E Galán
Jul 24, 2004·Infection and Immunity·Dan DrecktrahOlivia Steele-Mortimer

❮ Previous
Next ❯

Citations

Jul 23, 2013·The International Journal of Biochemistry & Cell Biology·Bahram KasmapourMaximiliano Gabriel Gutierrez
Aug 31, 2013·PloS One·Bernhard RoppenserJohn H Brumell
Oct 5, 2011·The Journal of Cell Biology·Cat M Haglund, Matthew D Welch
Aug 2, 2011·European Journal of Cell Biology·Roopa Rajashekar, Michael Hensel
Sep 10, 2008·Traffic·Malina A BakowskiJohn H Brumell
Sep 10, 2011·Cellular Microbiology·Terence A Agbor, Beth A McCormick
May 1, 2013·Emerging Microbes & Infections·Charlotte A Perrett, Daoguo Zhou
Nov 20, 2012·Cell Host & Microbe·Charlotte OdendallDavid W Holden
Jan 31, 2012·Virulence·Jan HänischKlemens Rottner
Mar 4, 2014·Current Opinion in Microbiology·Elizabeth A Creasey, Ralph R Isberg
Jan 23, 2009·Current Opinion in Microbiology·Emma J McGhieVassilis Koronakis
May 30, 2012·Biochemical and Biophysical Research Communications·Fernando Baisón-OlmoFrancisco Ramos-Morales
Jul 30, 2014·Frontiers in Chemistry·Venkaiah Betapudi
Jul 6, 2014·Pathogens and Disease·Mabel Lum, Renato Morona
Oct 29, 2014·Molecular & Cellular Proteomics : MCP·Stephanie VorwerkNicole Hansmeier
Jun 10, 2018·Cellular Microbiology·Kirsten C BoddyJohn H Brumell
Oct 24, 2017·Veterinary Sciences·Hilary K TruchanJason A Carlyon
Jan 14, 2021·International Journal of Molecular Sciences·Margaux Pillon, Patricia Doublet
Dec 18, 2020·Frontiers in Cellular and Infection Microbiology·Kuldeep Sachdeva, Varadharajan Sundaramurthy
May 6, 2021·Proceedings of the National Academy of Sciences of the United States of America·Marcel HahnIvan Dikic
Jun 24, 2016·Microbiology Spectrum·Gabriel MitchellDaniel A Portnoy
Jul 9, 2021·Cell Host & Microbe·Philipp WalchAthanasios Typas
Jun 17, 2009·Cell Host & Microbe·Yadunanda Kumar, Raphael H Valdivia

❮ Previous
Next ❯

Related Concepts

Related Feeds

CRISPR & Staphylococcus

CRISPR-Cas system enables the editing of genes to create or correct mutations. Staphylococci are associated with life-threatening infections in hospitals, as well as the community. Here is the latest research on how CRISPR-Cas system can be used for treatment of Staphylococcal infections.