Role for Nhp6, Gcn5, and the Swi/Snf complex in stimulating formation of the TATA-binding protein-TFIIA-DNA complex

Molecular and Cellular Biology
Debabrata BiswasDavid J Stillman

Abstract

The TATA-binding protein (TBP), TFIIA, and TFIIB interact with promoter DNA to form a complex required for transcriptional initiation, and many transcriptional regulators function by either stimulating or inhibiting formation of this complex. We have recently identified TBP mutants that are viable in wild-type cells but lethal in the absence of the Nhp6 architectural transcription factor. Here we show that many of these TBP mutants were also lethal in strains with disruptions of either GCN5, encoding the histone acetyltransferase in the SAGA complex, or SWI2, encoding the catalytic subunit of the Swi/Snf chromatin remodeling complex. These synthetic lethalities could be suppressed by overexpression of TOA1 and TOA2, the genes encoding TFIIA. We also used TFIIA mutants that eliminated in vitro interactions with TBP. These viable TFIIA mutants were lethal in strains lacking Gcn5, Swi2, or Nhp6. These lethalities could be suppressed by overexpression of TBP or Nhp6, suggesting that these coactivators stimulate formation of the TBP-TFIIA-DNA complex. In vitro studies have previously shown that TBP binds very poorly to a TATA sequence within a nucleosome but that Swi/Snf stimulates binding of TBP and TFIIA. In vitro binding experime...Continue Reading

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Citations

Jul 25, 2007·Molecular and Cellular Biology·Koji KasaharaTetsuro Kokubo
Jul 11, 2007·Molecular and Cellular Biology·Debabrata BiswasDavid J Stillman
Jul 1, 2005·Molecular and Cellular Biology·Debabrata BiswasDavid J Stillman
Jul 30, 2008·Experimental Cell Research·Akua K OduroFern E Murdoch
Jun 15, 2007·Biochimica Et Biophysica Acta·Torill HøibyHendrik G Stunnenberg
Mar 6, 2007·Mutation Research·Jeremy A Daniel, Patrick A Grant
Jun 28, 2014·Biochimica Et Biophysica Acta·Matias I HeppJosé L Gutiérrez
Aug 17, 2005·Current Opinion in Genetics & Development·Marco E Bianchi, Alessandra Agresti
Jun 5, 2013·Biochimica Et Biophysica Acta·Purnima Bhargava
Sep 16, 2006·The EMBO Journal·Debabrata BiswasDavid J Stillman
Oct 22, 2008·Molecular BioSystems·Tim Formosa
Apr 26, 2019·PloS One·Eszter BokorZsuzsanna Hamari
Oct 1, 2020·Nucleic Acids Research·Kiyoto KamagataReid C Johnson
Oct 3, 2020·Genes & Development·Jelly H M Soffers, Jerry L Workman

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