Role of 11-beta-hydroxysteroid dehydrogenase type 1 in differentiation of 3T3-L1 cells and in rats with diet-induced obesity

Acta Pharmacologica Sinica
Yun LiuGuo-xian Ding

Abstract

To observe the roles of 11-beta-hydroxysteroid dehydrogenase type 1 (11beta-HSD1) in in vitro preadipocyte differentiation and in rats with diet-induced obesity (DIO). Protein expression of 11beta-HSD1 in the process of 3T3-L1 cell differentiation and in various tissues of the rats were detected by Western blot analysis; expression of 11beta-HSD1 mRNA and glucocorticoid receptor (GR) and other marker genes of preadipocyte differentiation were detected by using real-time PCR. Lipid droplets in 3T3-L1 cells accumulated and increased after stimulation. A dramatically elevated protein level of 11beta-HSD1, especially in the late stages of 3T3-L1 cell differentiation, was detected. The relative mRNA levels of 11beta-HSD1, GR and cell differentiation markers LPL, aP2, and FAS were upregulated, and Pref-1 was downregulated during the differentiation. In DIO rats, bodyweight, visceral adipose mass index and the protein expression of 11beta-HSD1 increased, especially in adipose tissue, brain and muscles. Serum insulin, triglyceride, total cholesterol and low-density lipoprotein cholesterol were found to be increased in DIO rats, but without any obvious changes in blood glucose or tumor necrosis factor-alpha levels. 11beta-HSD1 may promo...Continue Reading

References

Aug 22, 1987·Lancet·G M Reaven, B B Hoffman
Dec 15, 1995·Cell·D J MangelsdorfR M Evans
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y KotelevtsevJ J Mullins
Feb 18, 1999·Molecular and Cellular Endocrinology·H M Reichardt, G Schütz
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·J L YauJ R Seckl
Jan 5, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Elizabeth H RabbittMartin Hewison
Jan 27, 2004·Obesity Research·Brian R Walker
Feb 26, 2004·Obesity Surgery·Nahid HamouiPeter F Crookes
Mar 18, 2004·Molecular and Cellular Endocrinology·Deborah J Wake, Brian R Walker
Dec 14, 2004·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·C AsensioF Rohner-Jeanrenaud
Jan 13, 2005·American Journal of Physiology. Endocrinology and Metabolism·Elena VelkoskaMargaret J Morris

❮ Previous
Next ❯

Citations

Aug 21, 2014·International Journal of Endocrinology·Nicola NapoliSol Epstein
Jul 13, 2007·Molecular and Cellular Endocrinology·Alexandros Vegiopoulos, Stephan Herzig
Sep 6, 2007·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Lan-Juan ZhaoHong-Wen Deng
Jul 22, 2014·Cell Biology International·Qiong HuangYun Liu
Sep 10, 2017·Apoptosis : an International Journal on Programmed Cell Death·Yoon-Ju NaKi Young Kim

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.