Role of 12-lipoxygenase in volatile anesthetic-induced delayed preconditioning in mice

American Journal of Physiology. Heart and Circulatory Physiology
Yasuo M TsutsumiDavid M Roth

Abstract

Delayed cardiac protection mediated by 12-lipoxygenase (12-LO) expression and activity has been linked to opioid receptor stimulation. The role of 12-LO in volatile anesthetic-induced delayed cardiac protection has not been determined. We tested the hypothesis that expression and activity of 12-LO mediate delayed cardiac protection induced by isoflurane in the mouse heart in vivo. Mice were pretreated with 1.4% isoflurane for 30 min and allowed to recover for 1, 12, or 24 h. Immunoblot analysis showed isoflurane significantly enhanced 12-LO protein expression at 12 and 24 h after isoflurane exposure, and this induction of 12-LO was confirmed by immunohistochemistry of whole heart sections at 24 h. The induced protein expression appeared to be localized to intercalated disc regions adjoining adjacent cardiac myocytes. Additionally, mice +/- isoflurane (24 h previously) were subjected to 30 min coronary artery occlusion followed by 2 h of reperfusion in the presence and absence of a 12-LO inhibitor. Isoflurane reduced infarct size (27.1 +/- 2.2% of the area at risk; n = 8) compared with the control group (44.6 +/- 3.6%, n = 8). Baicalein (3 mg/kg), a selective 12-LO inhibitor, blocked the delayed protective effects of isoflurane ...Continue Reading

References

Jan 1, 1986·Annual Review of Biochemistry·P NeedlemanJ B Lefkowith
Apr 14, 1982·Biochemical and Biophysical Research Communications·K Sekiya, H Okuda
May 1, 1997·Anesthesiology·J R KerstenD C Warltier
Aug 30, 2000·Proceedings of the National Academy of Sciences of the United States of America·K ShinmuraR Bolli
Apr 12, 2001·American Journal of Physiology. Heart and Circulatory Physiology·S A GabelE Murphy
Feb 9, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Zuo-Hui ShaoChun-Su Yuan
Sep 3, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hemal H PatelGarrett J Gross
Oct 18, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Eitaro KodaniRoberto Bolli
Feb 22, 2005·The Journal of Pharmacy and Pharmacology·Wen-Hsin HuangTz-Chong Chou
Oct 4, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ioana R PrestonBarry L Fanburg
Feb 25, 2006·Acta Physiologica·K B AnderssonG Christensen

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Citations

Nov 20, 2013·Apoptosis : an International Journal on Programmed Cell Death·Lina SongHui-Hua Li
Nov 27, 2010·Journal of Cardiothoracic and Vascular Anesthesia·Paul S Pagel, Judith A Hudetz
Sep 10, 2014·Cardiovascular Diabetology·Yasuo M TsutsumiKatsuya Tanaka
Sep 8, 2011·Current Protocols in Cell Biology·Vadim Zinchuk, Olga Grossenbacher-Zinchuk
Jun 14, 2008·Current Protocols in Cell Biology·Vadim Zinchuk, Olga Zinchuk
Aug 25, 2007·Anesthesia and Analgesia·Eliana LucchinettiMichael Zaugg
Nov 21, 2008·Anesthesia and Analgesia·Nina C WeberBenedikt Preckel
Mar 13, 2014·Current Protocols in Cell Biology·Vadim Zinchuk, Olga Grossenbacher-Zinchuk

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