Role of 1α,25-Dihydroxyvitamin D3 in Adipogenesis of SGBS Cells: New Insights into Human Preadipocyte Proliferation

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Ingrid FelicidadeLúcia Regina Ribeiro

Abstract

Compared with non-obese individuals, obese individuals commonly store more vitamin D in adipose tissue. VDR expression in adipose tissue can influence adipogenesis and is therefore a target pathway deserving further study. This study aims to assess the role of 1,25(OH)2D3 in human preadipocyte proliferation and differentiation. RTCA, MTT, and trypan blue assays were used to assess the effects of 1,25(OH)2D3 on the viability, proliferation, and adipogenic differentiation of SGBS cells. Cell cycle and apoptosis analyses were performed with flow cytometry, triglycerides were quantified, and RT-qPCR was used to assess gene expression. We confirmed that the SGBS cell model is suitable for studying adipogenesis and demonstrated that the differentiation protocol induces cell maturation, thereby increasing the lipid content of cells independently of treatment. 1,25(OH)2D3 treatment had different effects according to the cell stage, indicating different modes of action driving proliferation and differentiation. In preadipocytes, 1,25(OH)2D3 induced G1 growth arrest at both tested concentrations without altering CDKN1A gene expression. Treatment with 100 nM 1,25(OH)2D3 also decreased MTT absorbance and the lipid concentration. Moreover, ...Continue Reading

Citations

Feb 26, 2019·Diabetes/metabolism Research and Reviews·Adriyan PramonoEllen E Blaak
Dec 20, 2018·Nutrients·Luca Dalle CarbonareAngelo Pietrobelli
Feb 15, 2020·Nutrición hospitalaria·Hillary Kimberly Osorio LandaElizabet Sánchez Landa
Dec 8, 2020·Nutrition Research and Practice·Hataikarn NimitphongMi-Jeong Lee
Jun 8, 2021·Journal of Lipid and Atherosclerosis·Chan Yoon Park, Sung Nim Han

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