PMID: 6161640Nov 25, 1980Paper

Role of 2',5'-oligo(adenylic acid) polymerase in the degradation of ribonucleic acid linked to double-stranded ribonucleic acid by extracts of interferon-treated cells

Biochemistry
T W NilsenC Baglioni

Abstract

RNA covalently linked to double-stranded RNA (dsRNA) is preferentially degraded in extracts of interferon-treated HeLa cells [Nilsen, T. W., & Baglioni, C. (1979) Proc. Natl. Acad. Sci. U.S.A. 76, 2600-2604]. The size of the dsRNA required for this preferential degradation has been determined by annealing poly(I) of known length to the poly(C) tract of encephalomyocarditis virus (EMCV) RNA or by annealing poly(U) to poly(A) of known length of vesicular stomatitis virus mRNA. The dsRNA must be longer than about 60 base pairs to observe the preferential degradation of RNA. Moreover, triple-stranded regions that do not activate synthesis of 2',5'-oligo(A) and ethidium bromide, which intercalates in dsRNA and blocks 2',5'-olido(A) polymerase activation, prevent this degradation. Ethidium also blocks the degradation of the replicative intermediate of EMCV by extracts of interferon-treated cells. These experiments indicate that synthesis of 2',5'-oligo(A) is required for the degradation of RNA linked to dsRNA. The 2',5'-oligo(A)-dependent endonuclease does not cleave single- or double-stranded DNA, nor does it cleave homopolyribonucleotides. The potential role of the 2',5'-oligo(A) polymerase/endonuclease system in the inhibition of ...Continue Reading

References

Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A G HovanessianL Montagnier
Jun 1, 1975·Journal of Virology·D H Spector, D Baltimore
Jul 26, 1976·Biochemical and Biophysical Research Communications·K M Chumakov, V I Agol
Jun 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·T W Nilsen, C Baglioni
Sep 1, 1979·FEBS Letters·B R WilliamsI M Kerr
Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·I M Kerr, R E Brown
Sep 25, 1979·Journal of Molecular Biology·T Nilsen, C Baglioni
Jun 25, 1974·Journal of Molecular Biology·J GorskiJ B Lingrel
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·I Krishnan, C Baglioni
Feb 1, 1980·Virology·A G Hovanessian, J N Wood

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Citations

Jun 12, 1998·Pharmacology & Therapeutics·M R Player, P F Torrence
Jan 1, 1990·Microbiology and Immunology·A TominagaR Noyori
Dec 29, 2013·Nucleic Acids Research·Ao ZhangRobert H Silverman
Apr 3, 2007·Biochimie·Catherine Bisbal, Robert H Silverman
Nov 10, 1988·Biochimica Et Biophysica Acta·A Luxembourg

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