Role of 5-lipoxygenase in the multiple organ failure induced by zymosan

Intensive Care Medicine
Salvatore CuzzocreaL Sautebin

Abstract

This study investigated the role of 5-lipoxygenase in the pathogenesis of multiple organ failure (MOF) induced by zymosan. Male mice with a targeted disruption of the 5-lipoxygenase gene (5-LOKO) and littermate wild-type (WT) controls (5-LOWT) were used to evaluate the role of 5-lipoxygenase (5-LO) in the pathogenesis of MOF. University research laboratory. MOF was induced by peritoneal injection of zymosan (500 mg/kg i.p. as a suspension in saline) in 5-LOWT and in 5-LOKO mice. MOF was assessed 18 h after administration of zymosan and monitored for 12 days (for loss of body weight and mortality). A severe inflammatory process induced by zymosan administration in WT mice coincided with the damage of lung and small intestine, as assessed by histological examination. Myeloperoxidase activity indicative of neutrophil infiltration and lipid peroxidation were significantly increased in zymosan-treated WT mice. Zymosan in the WT mice also induced a significant increase in the plasma level of nitrite/nitrate. Immunohistochemical examination demonstrated a marked increase in the immunoreactivity to ICAM-1 and P-selectin in the lung and intestine of zymosan-treated WT mice. In contrast, the degree of (a) peritoneal inflammation and tiss...Continue Reading

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Citations

Feb 20, 2010·Journal of Inflammation·Maria GaluppoSalvatore Cuzzocrea
Feb 26, 2011·European Journal of Pharmacology·Stefania MondelloSalvatore Cuzzocrea
May 7, 2008·Pharmacology, Biochemistry, and Behavior·Masataka Saito, Shiro Watanabe
Mar 14, 2007·The Journal of Surgical Research·Cengiz BolcalHarun Tatar
Apr 26, 2005·Biochimica Et Biophysica Acta·Hisayo OkamotoOlof Rådmark
Dec 1, 2010·Current Therapeutic Research, Clinical and Experimental·Orhan Veli OzkanSadik Sogut
Jan 23, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Claire LastrucciCéline Cougoule
Jun 1, 2011·Free Radical Research·Daniela ImpellizzeriSalvatore Cuzzocrea

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