Role of a highly conserved electrostatic interaction on the surface of cytochrome C in control of the redox function

Biochemistry
Hulin TaiYasuhiko Yamamoto

Abstract

In Hydrogenobacter thermophilus cytochrome c(552), an electrostatic interaction between Lys8 and Glu68 in the N- and C-terminal helices, respectively, stabilizes its protein structure [Travaglini-Allocatelli, C., Gianni, S., Dubey, V. K., Borgia, A., Di Matteo, A., Bonivento, D., Cutruzzola, F., Bren, K. L., and Brunori, M. (2005) J. Biol. Chem. 280, 25729-25734], this electrostatic interaction being a highly conserved structural feature of the cytochrome c family. In the present study, the functional consequences of removal of the interaction through replacement of Lys8 by Ala have been investigated in order to elucidate the molecular mechanisms responsible for functional control of the protein. The mutation resulted in a decrease in protein stability, as reflected in lowering of the denaturation temperature by approximately 2-9 degrees C, and a negative shift by approximately 8 mV of the redox potential (E(m)) of the protein. The decrease in the protein stability was attributed to the enthalpic loss due to the removal of the intramolecular interaction. The negative shift of the E(m) value was shown to be due to the effect of the mutation on the entropic contribution to the E(m) value. The small, but subtle, effects of removal...Continue Reading

References

Nov 1, 1992·Journal of Biomolecular NMR·M PiottoV Sklenár
Sep 28, 1999·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·L BanciP Turano
May 23, 2000·Biochemistry·S L SpringsG L McLendon
Aug 5, 2000·The Journal of Biological Chemistry·J HasegawaY Igarashi
May 29, 2001·The Journal of Biological Chemistry·C Blouin, C J Wallace
Jan 5, 2002·Journal of the American Chemical Society·Gianantonio BattistuzziMarco Sola
Sep 26, 2002·Journal of the American Chemical Society·Yasuhiko YamamotoYasuo Igarashi
Sep 27, 2002·Current Opinion in Biotechnology·Bertus van den Burg, Vincent G H Eijsink
Nov 6, 2003·Journal of the American Chemical Society·Norifumi TeruiYasuhiko Yamamoto
Jul 28, 2004·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·G BattistuzziM Sola
Nov 13, 2004·Journal of the American Chemical Society·Susumu UchiyamaYuji Kobayashi
May 11, 2005·The Journal of Biological Chemistry·Carlo Travaglini-AllocatelliMaurizio Brunori
Nov 13, 2008·Proteins·Zhong Zheng, M R Gunner
Mar 19, 2009·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Shin-ichi J TakayamaYasuhiko Yamamoto

❮ Previous
Next ❯

Citations

Aug 30, 2011·The Journal of Physical Chemistry. B·Benjamin D LevinSean J Elliott
Feb 19, 2011·Chemical Communications : Chem Comm·Huan LiuShuji Nakanishi
Apr 26, 2013·Cellular and Molecular Life Sciences : CMLS·Sobia ZaidiFaizan Ahmad
May 22, 2021·Physical Chemistry Chemical Physics : PCCP·Elizabeth A Karnaukh, Ksenia B Bravaya
Oct 14, 2017·Chemical Reviews·Damián Alvarez-PaggiDaniel H Murgida

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.