Role of a peptide tagging system in degradation of proteins synthesized from damaged messenger RNA

Science
K C KeilerR T Sauer

Abstract

Variants of lambda repressor and cytochrome b562 translated from messenger RNAs without stop codons were modified by carboxyl terminal addition of an ssrA-encoded peptide tag and subsequently degraded by carboxyl terminal-specific proteases present in both the cytoplasm and periplasm of Escherichia coli. The tag appears to be added to the carboxyl terminus of the nascent polypeptide chain by cotranslational switching of the ribosome from the damaged messenger RNA to ssrA RNA.

References

Jul 2, 1979·Molecular & General Genetics : MGG·B K Ray, D Apirion
Dec 1, 1992·Microbiological Reviews·S Gottesman, M R Maurizi
Sep 1, 1991·Molecular & General Genetics : MGG·B K Oh, D Apirion
Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·K R SilberR T Sauer
Dec 5, 1991·European Journal of Biochemistry·H NikkilaS G Sligar
Feb 1, 1990·Genes & Development·D A ParsellR T Sauer
Dec 1, 1995·The Journal of Biological Chemistry·K C Keiler, R T Sauer
Sep 27, 1994·Proceedings of the National Academy of Sciences of the United States of America·Y KomineH Inokuchi
Apr 1, 1995·Current Opinion in Cell Biology·M Hochstrasser
Oct 7, 1994·Cell·A Ciechanover
Apr 1, 1994·Journal of Bacteriology·D M RetallackD I Friedman
Aug 1, 1995·Protein Science : a Publication of the Protein Society·K C KeilerR T Sauer
Feb 2, 1996·The Journal of Biological Chemistry·K C Keiler, R T Sauer

❮ Previous
Next ❯

Citations

May 20, 1999·Biopolymers·C W HilbersH A Heus
Sep 5, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Shobha VasudevanCarol J Wilusz
Dec 1, 1996·Plant Molecular Biology·Z Adam
Oct 1, 1996·Plant Molecular Biology·R D Vierstra
Oct 12, 2007·Histochemistry and Cell Biology·Sadaki YokotaToshiaki Oda
Nov 13, 2008·Systems and Synthetic Biology·Michael S AllenMichael L Simpson
Nov 26, 2003·Gene·Roderick A Slavcev, Sidney Hayes
Mar 24, 2004·Journal of Structural Biology·Axel MogkBernd Bukau
Mar 24, 2004·Journal of Structural Biology·Philip Wong, Walid A Houry
Apr 23, 2004·Current Opinion in Structural Biology·Peter W HaebelNenad Ban
Jul 4, 2013·Trends in Biochemical Sciences·Emmanuel Giudice, Reynald Gillet
Jan 29, 2000·Journal of Immunological Methods·C SchaffitzelA Plückthun
Sep 10, 2002·Journal of Molecular Biology·Diane O'Handley, Hiroshi Nakai
Jul 9, 1999·Biochimica Et Biophysica Acta·B FeldenJ F Atkins
Jan 1, 1996·Biochimie·B FeldenR F Gesteland
Apr 12, 2002·Gene·Pavel V BaranovJohn F Atkins
Oct 1, 2003·Biotechnology Advances·Kerrm Y F YauJ Christopher Hall
Sep 18, 2002·Research in Microbiology·James A TriccasWarwick J Britton
Oct 6, 1999·Current Opinion in Biotechnology·F Baneyx
Jul 17, 1998·Current Opinion in Structural Biology·R GiegéJ Rudinger
Mar 19, 1999·Trends in Microbiology·R De MotW Baumeister
Jul 1, 1996·Trends in Biochemical Sciences·M Rechsteiner, S W Rogers
Nov 1, 1996·Trends in Biochemical Sciences·M J Ruiz-EchevarriaS W Peltz
Aug 4, 1999·Trends in Biochemical Sciences·Y M Hou
Jun 1, 1997·Bioorganic & Medicinal Chemistry·J RudingerR Giegé
Dec 10, 2002·Current Opinion in Chemical Biology·Alexander HüttenhoferJean Pierre Bachellerie
Apr 6, 2002·Current Opinion in Microbiology·Jeffrey H Withey, David I Friedman
Mar 6, 1999·Current Opinion in Microbiology·C Herman, R D'Ari
May 14, 1999·Current Opinion in Microbiology·S Gottesman

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.