Role of a rat membrane inhibitor of complement in anti-basement membrane antibody-induced renal injury

Kidney International
Y HatanakaS Matsuo

Abstract

In the kidneys of anti-glomerular basement membrane (anti-GBM) antibody disease, binding of antibodies to tubular basement membrane (TBM) is often observed. The present work was performed to explore the mechanisms of binding of anti-GBM antibodies to TBM in vivo with special reference to 5I2Ag, a rat membrane inhibitor of complement which regulates complement activation at C3 convertase level. To suppress functions of renal 5I2Ag, F(ab')2 fragment of 5I2 (a neutralizing mAb against 5I2Ag) was perfused in the left kidney and then blood circulation was restored. Mild proteinuria ( < 10 mg/16 hr) was observed during first several days. Five days later, there were tubulointerstitial injuries defined by tubular vimentin staining and leukocyte infiltration. Significant deposition of C3 was observed in the capillaries and in TBM. In rats intravenously injected with rabbit anti-rat GBM antibodies five minutes after kidney perfusion with 5I2, strong binding of rabbit IgG to TBM was observed at one and five days after injection. Although these rats showed mild proteinuria comparable to those perfused with 5I2 and those injected with normal rabbit serum, tubulointerstitial injury was significantly enhanced at Day 5. In contrast, rats perf...Continue Reading

References

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Citations

Nov 21, 1998·Immunology·K NishikawaH Okada
Dec 9, 1998·Kidney International·M Nangaku
Jul 19, 2005·Expert Opinion on Investigational Drugs·Masashi Mizuno, Duncan S Cole
Mar 6, 2007·Seminars in Nephrology·Lihua Bao, Richard J Quigg
Aug 15, 2006·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Uri Galili
Jan 19, 2007·Journal of the American Society of Nephrology : JASN·Lihua BaoRichard J Quigg
Feb 22, 2003·Journal of the American Society of Nephrology : JASN·William G Couser
Jan 3, 2001·Journal of the American Society of Nephrology : JASN·Brigitte SchillerRichard J Quigg
Sep 8, 2000·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·P PaueksakonA B Fogo

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