Role of a single noncoding nucleotide in the evolution of an epidemic African clade of Salmonella

Proceedings of the National Academy of Sciences of the United States of America
Disa L HammarlöfJay C D Hinton

Abstract

Salmonella enterica serovar Typhimurium ST313 is a relatively newly emerged sequence type that is causing a devastating epidemic of bloodstream infections across sub-Saharan Africa. Analysis of hundreds of Salmonella genomes has revealed that ST313 is closely related to the ST19 group of S Typhimurium that cause gastroenteritis across the world. The core genomes of ST313 and ST19 vary by only ∼1,000 SNPs. We hypothesized that the phenotypic differences that distinguish African Salmonella from ST19 are caused by certain SNPs that directly modulate the transcription of virulence genes. Here we identified 3,597 transcriptional start sites of the ST313 strain D23580, and searched for a gene-expression signature linked to pathogenesis of Salmonella We identified a SNP in the promoter of the pgtE gene that caused high expression of the PgtE virulence factor in African S. Typhimurium, increased the degradation of the factor B component of human complement, contributed to serum resistance, and modulated virulence in the chicken infection model. We propose that high levels of PgtE expression by African S Typhimurium ST313 promote bacterial survival and dissemination during human infection. Our finding of a functional role for an extrage...Continue Reading

References

Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P I FieldsF Heffron
Jan 1, 1972·Molecular & General Genetics : MGG·H Schmieger
Apr 11, 1993·Nucleic Acids Research·S Lisser, H Margalit
May 18, 2000·Microbes and Infection·R Rautemaa, S Meri
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 30, 2002·Nucleic Acids Research·Arthur L DelcherSteven L Salzberg
Oct 1, 1952·The Journal of Pathology and Bacteriology·G LUMB, W BEAUTYMAN
Aug 6, 2004·International Journal of Medical Microbiology : IJMM·Maini Kukkonen, Timo K Korhonen
Mar 12, 2005·Cellular Microbiology·Kaarina LähteenmäkiTimo K Korhonen
Jun 6, 2006·Gene·Simanti DattaDonald L Court
Sep 25, 2007·International Journal of Medical Microbiology : IJMM·Päivi RamuKaarina Lähteenmäki
Aug 6, 2008·The Journal of Biological Chemistry·Haowei SongYixin Shi
Apr 21, 2009·Methods in Molecular Biology·Stéphane GuindonOlivier Gascuel
Jun 6, 2009·Bioinformatics·Samuel AssefaMatthew Berriman
Sep 15, 2009·PLoS Computational Biology·Steve HoffmannJörg Hackermüller
Nov 13, 2009·Proceedings of the National Academy of Sciences of the United States of America·M Analise ZaunbrecherJames E Posey
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Esther N GondweCalman A MacLennan
Aug 14, 2010·Journal of Thrombosis and Haemostasis : JTH·M Valls SerónJ C M Meijers
Jan 11, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Matthew K SigginsCalman A MacLennan
Apr 22, 2011·Molecular Microbiology·Sanna KoskiniemiDan I Andersson
Apr 28, 2012·Proceedings of the National Academy of Sciences of the United States of America·Carsten KrögerJay C D Hinton
Aug 9, 2012·Theory in Biosciences = Theorie in Den Biowissenschaften·Günter P WagnerVincent J Lynch
Oct 23, 2012·Journal of Immunological Methods·Yun Shan Goh, Calman A MacLennan
Apr 5, 2013·Clinical Microbiology Reviews·Anna Fàbrega, Jordi Vila
Apr 26, 2013·Theory in Biosciences = Theorie in Den Biowissenschaften·Günter P WagnerVincent J Lynch

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Citations

May 23, 2018·Infection and Immunity·Priscilla BranchuRobert A Kingsley
May 21, 2019·Microbial Genomics·Jane HawkeyFrançois-Xavier Weill
Jun 25, 2019·PLoS Genetics·Keith D MacKenzieAaron P White
Feb 6, 2020·Microbial Genomics·Siân V OwenJay C D Hinton
Apr 15, 2020·PLoS Neglected Tropical Diseases·Marcela Suárez-EsquivelCaterina Guzmán-Verri
Jul 22, 2020·Human Vaccines & Immunotherapeutics·Giulia Piccini, Emanuele Montomoli
Mar 15, 2020·FEBS Letters·Eric S Krukonis, Joshua J Thomson
Aug 14, 2020·Proceedings of the National Academy of Sciences of the United States of America·Lorena Preciado-LlanesAlison Simmons
Nov 21, 2019·Indian Journal of Medical Microbiology·Dhiviya Prabaa Muthuirulandi SethuvelVeeraraghavan Balaji
Dec 19, 2018·Proceedings of the National Academy of Sciences of the United States of America·Elizabeth J KlemmGordon Dougan
Sep 5, 2020·Journal of Drug Targeting·Lance B AugustinJanet L Schottel
Oct 13, 2020·Nucleic Acids Research·Jeongjoon Choi, Eduardo A Groisman
Aug 19, 2020·Trends in Microbiology·Anne-Béatrice Blanc-Potard, Eduardo A Groisman
Apr 1, 2019·Indian Journal of Medical Microbiology·Dhiviya Prabaa Muthuirulandi SethuvelVeeraraghavan Balaji
Oct 24, 2020·International Journal of Molecular Sciences·Marcela Suárez-EsquivelCaterina Guzmán-Verri
Apr 7, 2019·Microbiology Spectrum·Amy YeungGordon Dougan
Jan 19, 2019·EcoSal Plus·James J Gilchrist, Calman A MacLennan
Jun 28, 2021·Current Opinion in Microbiology·Mark P Stevens, Robert A Kingsley
Jul 1, 2021·Microbiology and Molecular Biology Reviews : MMBR·Eduardo A GroismanJeongjoon Choi
Sep 15, 2018·Microbiology Spectrum·Blanca M Perez-Sepulveda, Jay C D Hinton

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Datasets Mentioned

BETA
CP002487

Methods Mentioned

BETA
RNA-seq
dRNA-seq
flow cytometry
Illumina Sequencing
PCR
Assay
cleavage assay

Software Mentioned

Segemehl
Abacus
BLASTn
FlowJo
NUCmer
A5
PhylML
Integrated Genome Browser ( IGB )
PanSeq package

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