Role of a TPA-responsive element in hepcidin transcription induced by the bone morphogenetic protein pathway

Biochemical and Biophysical Research Communications
Yohei KanamoriMasayuki Funaba

Abstract

Systemic iron balance is governed by the liver-derived peptide hormone hepcidin. The transcription of hepcidin is primarily regulated by the bone morphogenetic protein (BMP) and inflammatory cytokine pathways through the BMP-response element (BMP-RE) and STAT-binding site, respectively. In addition to these elements, we previously identified a TPA-responsive element (TRE) in the hepcidin promoter and showed that it mediated the transcriptional activation of hepcidin through activator protein (AP)-1 induced by serum. In the present study, we examined the role of TRE in the BMP-induced transcription of hepcidin in HepG2 liver cells. The serum treatment increased the basal transcription of hepcidin; however, responsiveness to the expression of ALK3(QD), a constitutively active BMP type I receptor, was unaffected. Consistent with these results, mutations in TRE in the hepcidin promoter decreased basal transcription, whereas responsiveness to the expression of ALK3(QD) remained unchanged. HepG2 cells significantly expressed AP-1 components in the basal state, whereas BMP did not up-regulate the expression of these components. The expression of c-fos enhanced the basal transcription of hepcidin as well as ALK3(QD)-mediated hepcidin t...Continue Reading

References

May 4, 2002·Nature Cell Biology·Eitan Shaulian, Michael Karin
Jul 15, 2003·Molecular Biology of the Cell·Gyo MurakamiTakeshi Imamura
Nov 27, 2004·Journal of Cell Science·Jochen HessMarina Schorpp-Kistner
Jul 13, 2006·Blood·Diedra M Wrighting, Nancy C Andrews
Nov 21, 2007·Nature Chemical Biology·Paul B YuRandall T Peterson
Nov 26, 2008·Nature Medicine·Paul B YuKenneth D Bloch
Mar 3, 2009·Nature Genetics·Billy AndriopoulosJodie L Babitt
Apr 2, 2009·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Masaru MurakamiMasayuki Funaba
Nov 17, 2009·International Journal of Hematology·Junya KandaHiroshi Kawabata
Jul 7, 2010·Cell·Matthias W HentzeClara Camaschella
Jul 24, 2012·The Journal of Biological Chemistry·Sung-Young LeeZigang Dong
Nov 10, 2013·Blood·Delphine MeynardHerbert Y Lin
Aug 1, 2014·Frontiers in Pharmacology·Manfred NairzGünter Weiss

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Citations

Dec 7, 2016·Scientific Reports·Yohei KanamoriMasayuki Funaba

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