PMID: 29292Aug 1, 1978

Role of a transmembrane pH gradient in epinephrine transport by chromaffin granule membrane vesicles

Proceedings of the National Academy of Sciences of the United States of America
S SchuldinerB I Kanner

Abstract

ATP-driven transport and accumulation of epinephrine in chromaffin granule membrane vesicles isolated from bovine adrenal medulla is inhibited by the proton ionophores carbonylcyanide p-trifluoromethoxyphenylhydrazone and nigericin, but not by valinomycin. Moreover, an artificially imposed pH gradient (interior acid) is able to drive this reserpine-sensitive transport system in the absence of ATP. Dicyclohexylcarbodiimide, an inactivator of the chromaffin granule membrane-bound ATPase, completely inhibits ATP-dependent epinephrine accumulation, but has much less effect when an imposed pH gradient is the driving force for epinephrine transport. The findings provide a strong indication that a pH gradient (interior acid) is the immediate driving force for epinephrine uptake in these storage granules and suggest that ATP-driven epinephrine transport is the result of two processes: (i) generation of a proton electrochemical gradient (interior acid and positive) by the membrane-bound, proton-translocating ATPase; and (ii) pH gradient-driven accumulation of the catecholamine.

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Citations

Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S RamosH R Kaback
Jan 1, 1976·Neuroscience·C L BashfordG A Ritchie
Jan 1, 1977·FEBS Letters·T Flatmark, O C Ingebretsen
Jan 15, 1975·FEBS Letters·C L BashfordG A Ritchie
Jul 1, 1962·The Journal of Biological Chemistry·N KIRSHNER

Related Concepts

Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone
Catecholamine [EPC]
Adenosine Triphosphatases
Valinomycin
Alcoholic Intoxication, Chronic
Bos taurus
Pandavir
DNAH8
Integral to Membrane
Epinephrine Transport

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