Role of a xyloglucan-specific endo-beta-1,4-glucanase inhibitor in the interactions of Nicotiana benthamiana with Colletotrichum destructivum, C. orbiculare or Pseudomonas syringae pv. tabaci.

Molecular Plant Pathology
W XieP H Goodwin

Abstract

A xyloglucan-specific endo-beta-1,4-glucanase inhibitor cDNA, NbXEGIP1, was amplified from diseased leaves of Nicotiana benthamiana. The sequence was similar to the tomato xyloglucan-specific endo-beta-1,4-glucanase inhibitor (XEGIP) and tobacco nectarin IV genes that have been described as binding and inactivating fungal Family 12 xyloglucan-specific endo-beta-1,4-glucanases. Expression of NbXEGIP1 was not detected in healthy leaves, but the gene was induced during the later stages of infection by the fungi Colletotrichum destructivum and C. orbiculare. Induction of NbXEGIP1 also occurred during disease development by the bacterium Pseudomonas syringae pv. tabaci and during the hypersensitive response produced by P. syringae pv. tabaci expressing avrPto. A portion of NbXEGIP1 was cloned into a tobacco rattle virus vector for virus-induced gene silencing in N. benthamiana. Silencing NbXEGIP1 did not affect the interactions with either Colletotrichum species but did significantly reduce population levels of P. syringae pv. tabaci in the compatible interaction and P. syringae pv. tabaci expressing avrPto in the incompatible interaction. In the susceptible response to P. syringae pv. tabaci, silencing of NbXEGIP1 also resulted in ...Continue Reading

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Citations

Apr 9, 2013·Phytochemistry·Olivier HabryloVincent Phalip
Mar 15, 2012·Science China. Life Sciences·Changjun HuangXueping Zhou

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