PMID: 2001409Feb 19, 1991

Role of ABH blood group antigens in the stimulation of a DIDS-sensitive Ca2+ influx pathway in human erythrocytes by Ulex europaeus agglutinin I and a monoclonal anti A1 antibody

Biochimica Et Biophysica Acta
B EngelmannJ Duhm

Abstract

Of eleven agglutinating lectins tested, only one, Ulex europaeus agglutinin I (UEA1), stimulated Ca2+ uptake in quin2-loaded erythrocytes by about 2-fold. UEA1 is known to be an alpha-L-fucose and ABH blood group specific lectin. The 45Ca2+ influx induced by UEA1 was absent in the presence of extracellular fucose (5 and 15 mM) and depended on the ABH blood group of the donor, the stimulatory potency of the lectin decreasing in the order H greater than A2 greater than A1. Ca2+ entry blockers, such as cobalt and verapamil, did not affect the 45Ca2+ influx induced by UEA1. 4,4'-Diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS) inhibited dose-dependently with a Ki of 1-2 microM. 10 microM DIDS, 10 microM 4,4'-dinitrostilbene-2,2'-disulfonic acid (DNDS) and 20 microM dipyridamole fully blocked the 45Ca2+ influx induced by UEA1. The effect of UEA1 on 45Ca2+ influx was absent in K+ and Mg2+ media and was less pronounced in choline than in Na+ media. The 45Ca2+ influx induced by the lectin was abolished by preincubation with 12-O-tetradecanoylphorbol 13-acetate (TPA, 60 ng/ml). A monoclonal antibody raised against A1 erythrocytes (Bric 54) accelerated 45Ca2+ influx in quin2 loaded A1 erythrocytes by about 2-fold. No effect was seen ...Continue Reading

References

May 3, 1976·Biochemical and Biophysical Research Communications·R Averdunk
Nov 1, 1988·Biochimie·W M WATKINSP H Johnson
Dec 7, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jean-Claude BéïqueRichard L Huganir

Citations

Aug 1, 1992·Pflügers Archiv : European journal of physiology·B EngelmannJ Duhm
Jun 1, 1993·Comparative Biochemistry and Physiology. Comparative Physiology·B Engelmann, U Schumacher
Feb 3, 1992·Neuroscience Letters·F AngensteinR Jork
Sep 1, 2007·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Philipp AttanasioFlorian Lang

Related Concepts

Quin2
Ulex europaeus II lectin
Blood Group H Type 1 Antigen
Aminoquinolines
Monoclonal Antibodies
Calcium
Erythrocytes
Fluorochromes
Potassium
Rubidium

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